The objective of the present prospective pharmacokinetic study was to describe the variability of plasma gentamicin concentrations in critically ill patients with acute kidney injury (AKI) necessitating extended daily diafiltration (EDD-f) using a population pharmacokinetic model and to subsequently perform Monte Carlo dosing simulations to determine which dose regimen achieves the pharmacodyna...

The enzyme β-galactosidase catalyzes the hydrolysis of lactose into glucose and galactose, although for its effective application it is necessary to establish techniques purification, concentration, or polishing, such as membrane separation processes, in particular ultrafiltration. present study aimed investigate ultrafiltration diafiltration applied initial steps concentration salt removal, re...

we studied the expression of human growth hormone (hgh) in e. coli under a bacteriophage t5-base promoter in a pqe30 expression vector. for an efficient expression of hgh cdna, a number of codons at the hgh n-terminal coding region were altered based on the e. coli major codons. an over-expression of hgh in the bacteria, carrying the recombinant plasmids, was observed at 37°c in the presence of...

This study aimed to purify and partially characterize a keratinolytic protease produced by Bacillus sp. P45 through bioconversion of feather meal. Crude extract was purified using sequence an aqueous two-phase system (ATPS) in large volume systems (10, 50, 500 g) increase obtaining enzyme, followed diafiltration (DF) step. Purified characterized terms protein profile analysis SDS-PAGE, optimum ...

The object of research is the secondary material resources processing plant and animal raw materials, namely fruit pomace milk whey. One most problematic areas that waste these industries has high rates biological chemical oxygen consumption wastewater, which significantly impairs operation local city treatment facilities. In addition, parameters extraction determination quality indicators obta...

We studied the expression of human growth hormone (hGH) in E. coli under a bacteriophage T5-base promoter in a pQE30 expression vector. For an efficient expression of hGH cDNA, a number of codons at the hGH N-terminal coding region were altered based on the E. coli major codons. An over-expression of hGH in the bacteria, carrying the recombinant plasmids, was observed at 37°C in the presence of...

Filters used in continuous renal replacement therapy (CRRT) induce elimination by filtration, dialysis, and adsorption. The worldwide ST150® filter adsorbs cytokines. However, adsorption is a non-specific process which might alter the pharmacokinetics of drugs. Pharmacodynamic/pharmacokinetic relationship aminoglycosides evidences importance peak concentration at first dose. We hypothesize an v...

Supplying SARS-COVID-19 vaccines in quantities to meet global demand has a bottleneck manufacturing capacity. Assessment of existing mRNA (messenger ribonucleic acid) vaccine processing shows the need for digital twins enabled by process analytical technology approaches improve transfers capacity multiplication, reduction out-of-specification batch failures, qualified personnel training faster ...