Simultaneous turnover studies with radioiodine-labelled IgM and IgG were made in 12 patients with Crohn's disease. Intestinal protein loss was estimated by means of 59Fe-labelled iron dextran. The serum levels of IgM, IgG, and IgA were normal in most cases. The catabolic rate of IgM was increased in all but one case. A positive correlation was present between the catabolic rate and serum concen...

apparent. Instead we found a broad distribution of fluorescent power. To ascertain the fluorescent power corresponding to one Dil-LDL-receptor complex, we attached labelled LDL particles to a polylysine-coated slide and imaged under identical conditions to the cells. The fluorescent power histogram is shown in Fig. 1( b). The histogram shows a long-tailed distribution; this is at variance with ...

Monostructured single-wall carbon nanotubes (SWCNTs) are important in both scientific research and electronic and biomedical applications; however, the bulk separation of SWCNTs into populations of single-chirality nanotubes remains challenging. Here we report a simple and effective method for the large-scale chirality separation of SWCNTs using a single-surfactant multicolumn gel chromatograph...

The interaction between Streptococcus mutans dextransucrase (EC 2.4.1.5) and high-molecular-weight dextran was studied in both the presence and absence of substrate sucrose. Equivalent weight-percent solutions of primer dextrans that differed 200-fold in molecular weight were found to be equally efficient in priming new dextran synthesis. Sodium borohydride reduction of dextran had no effect on...

pended upon the quantity of the maltose and the dextran added as well as the average molecular weight of the dextran added. The combination of all three factors yielded dextran with a relative viscosity as low as 1.6. The decrease in viscosity occurred in an orderly manner. For the recovery of dextran of clinical size, only the addition of dextran was of any value. A yield of 34.6 per cent of t...

The therapeutic effects of saccharolytic and proteolytic enzymes were investigated in models of IgA nephropathy. Mesangial glomerulonephritis was induced in mice by intravenous injection of preformed soluble immune complexes of dextran sulfate and either IgA (J 558) or IgM (MOPC 104 E) antidextran MAb (passive model) or by immunization with DEAE dextran (active model). In the passive model, onl...

Dextran sulfate is commonly used with polyethylene glycol to concentrate viruses before extraction of their DNA. However, dextran slulfate then easily contaminated such DNA and acted as a potent inhibitor of DNA polymerases from Bacillus subtilis (III), phage PBS2, and phage T4. Dextran sulfate only weakly inhibited Micrococcus luteus and Escherichia coli DNA polymerase I preparations.

Iron-dextran complex has been found to be carcinogenic in animals. Richmond (9, 10), Haddow (3), and Haddow and Horning (4) have reported that subcutaneous injections of massive doses of this complex in the rat and mouse have given rise to sarcomas originating from iron-laden connective and reticuloendothelial tissues. It was thought that it would be interesting to grow the mouse subcutaneous f...

Rheological tests performed under conditions relevant to those experienced during proof and oven rise are necessary for understanding the mechanisms of dextran addition on wheat dough baking performance. This study evaluated effect a high molecular weight (Mw) dextran, produced in situ by Weissella confusa A16 or externally added, rheological properties including, (i) proofing behavior using ma...

For antitumor agents introduced directly into the intracranial space, the extent of penetration into tissue, and hence the effectiveness of therapy, depends on the rate of drug elimination from the tissue. To test the hypothesis that slowly eliminated agents would penetrate further through tissues, methotrexate (MTX)-dextran conjugates were produced by covalently linking MTX to dextran through ...