Sucrose density gradient centrifugation has been used to measure the binding of Triton X-100 above its critical micellar concentration to a variety of purified membrane and non-membrane proteins. In addition, binding studies were done on the three proteins below the critical micellar concentration of detergent to distinguish between the interaction of proteins with detergent monomers and deter...

The α-pore-forming toxin Cytolysin A (ClyA) is responsible for the hemolytic activity of various Escherichia coli and Salmonella enterica strains. Soluble ClyA monomers spontaneously assemble into annular dodecameric pore complexes upon contact with membranes or detergent. At ClyA monomer concentrations above ∼100 nm, the rate-limiting step in detergent- or membrane- induced pore assembly is th...

Neurotoxic esterase (NTE) is a membrane-bound protein found in highest concentration in brain and lymphocytes. The enzyme has no known physiological function, but its organophosphorylation and aging in neural tissue are thought to trigger the pathogenesis of organophosphorus-induced delayed neuropathy (OPIDN). Solubilization of NTE from microsomal membranes from hen or chick brain was studied w...

the scope for using sunflower cake in ration formulation was tested in 6 horses employed for equine tourism. three experimental diets providing the same amount of proteins and energy as the control diet were formulated using rising doses of sunflower cake (sd3: 0.3 kg; sd6: 0.6 kg and sd10: 1.0 kg). digestibility evaluation using internal markers showed that rising sunflower cake doses reduced ...

G protein-coupled receptors (GPCRs) are the largest class of pharmaceutical protein targets, yet drug development is encumbered by a lack of information about their molecular structure and conformational dynamics. Most mechanistic and structural studies as well as in vitro drug screening with purified receptors require detergent solubilization of the GPCR, but typically, these proteins exhibit ...

The effects of different additives, including lactic acid bacteria inoculants, acremonium cellulase and mixtures of lactic acid bacteria inoculants and acremonium cellulase on changes of nutritive components and in vitro digestibility of Ceratoides arborescens during ensiling were investigated. The silages of each treatment were prepared in plastic film bags, kept in an incubator at 30°C and ba...

Reconstitution of integral membrane proteins (IMP) in aqueous solutions of detergent micelles has been extensively used in structural biology, using either X-ray crystallography or NMR in solution. Further progress could be achieved by establishing a rational basis for the selection of detergent and buffer conditions, since the stringent bottleneck that slows down the structural biology of IMPs...

We found that a mutant, Bcl-X(L)(F131V), which was previously reported to have impaired binding capacity, can bind to Bax almost as strongly as wild-type Bcl-X(L). In the absence of detergent, the Bcl-X(L)(F131V) mutant adopts the same conformation as wild-type Bcl-X(L), as determined by circular dichroism spectroscopy and size-exclusion chromatography. However, non-ionic detergent induces a co...

phase domains, leaving the lo-phase domains relatively unchanged as DRMs. Both of these approaches showed that TX100 did not substantially change the relative amounts of membrane in the two phases. By contrast, using calorimetry, Heerklotz and colleagues reported that TX100 can induce phase separation in previously uniform mixtures (45, 46). However, results of the other two studies described a...

The chemical composition, in situ  rumen degradability coefficients of dry matter (DM) and crude protein (CP), in vitro gas production and in vivo  digestibility were evaluated for olive cake (OC) silage treated (DM basis) with different additives as follows: (1) untreated OC; (2) OC, 8% molasses and 0.4% formic acid and (3) OC, 8% molasses, 0.4% formic acid and 0.5% urea. After addition of the...