Aggravation of atopic dermatitis (AD) is closely associated with excessive production cytokines by macrophages. To investigate the mechanisms how macrophages in human skin sense extracellular environment will help to understand diseases, however details remain unclear. In this study, we searched expression pattern 27 transient receptor potential (TRP) channels immune cells using protein atlas d...

Accumulation of lipid-laden foam cells of monocyte origin plays an important role in atherogenesis. Therefore, for determination of the mechanism of accelerated atherogenesis in Werner's syndrome, studies were carried out on the metabolism of acetylated low density lipoprotein (LDL) by monocyte-derived macrophages from patients with this syndrome. These macrophages showed abnormally high activi...

The present study examines the effects of water soluble extracts of gas-phase cigarette smoke on intracellular transglutaminase activities of intact, murine, bone marrow-derived macrophages maintained in culture. Western blotting of cell lysates utilizing noncross-reactive antisera indicate that mouse bone marrow-derived macrophages contain both tissue-type transglutaminase and factor XIII-asso...

BACKGROUND There are a few works considering the characterization of canine monocyte-derived macrophages as well as a standardized procedure for isolation, culture, and infection of these cells with Leishmania. We have performed several modifications in order to improve the canine monocyte-derived macrophage cultures. In addition, we have done a comparative study between monocytes and monocyte-...

Sialoadhesin (Sn, Siglec-1, CD169) is a member of the sialic acid binding Ig-like lectin (siglec) family expressed on macrophages. Its macrophage specific expression makes it an attractive target for delivering antigens to tissue macrophages via Sn-mediated endocytosis. Here we describe a novel approach for delivering antigens to macrophages using liposomal nanoparticles displaying high affinit...

BACKGROUND Although inflammation and prostate cancer (PCa) have been linked, the molecular interactions between macrophages and PCa cells are poorly explored. Pigment Epithelium-Derived Factor (PEDF) is an anti-angiogenic and anti-tumor factor. We previously showed that PEDF induces macrophages recruitment in vitro, correlates with macrophages density in human prostate, and stimulates macrophag...

activated macrophages have been classified into classical (m1) and alternative (m2) macrophages. we aimed to establish a method to yield enough number of macrophages to analyze their molecular, biological and immunological functions. we used drugs; adjuvant albumin from chicken egg whites—imject alum (ova- alum) and ova complete freund adjuvant (ova-cfa), to induce macrophages to m2 and m1 resp...

objective(s) the fractions of candida albicans have been used as an immunomodulator. the present work assessed the effect of different fractions of c. albicans on nitric oxide (no) production by mice peritoneal macrophages. materials and methods cell wall and cytoplasmic fractions of c. albicans atcc 10321 strain were extracted. mice peritoneal macrophages were purified and cultured. different ...

The death of retinal ganglion cells (RGCs) is a hallmark of many retinal neuropathies. Neuroprotection, axonal regeneration, and cell renewal are vital for the integrity of the visual system after insult but are scarce in the adult mammalian retina. We hypothesized that monocyte-derived macrophages, known to promote healing in peripheral tissues, are required after an insult to the visual syste...

Monocytes and macrophages belong to the mononuclear phagocyte system (MPS). The concept of the MPS is based on the idea that circulating monocytes represent the immediate precursors of tissue macrophages. Recent genetic fate mapping of myeloid cells caused a conceptual frameshift, as it was found that many macrophages are derived from embryonic precursors that seed the tissues before birth and ...