In the present research, nanoindentation, atomic-force microscopy and optical were used to study mechanical microgeometrical parameters of tooth tissues. A nanoindentation test unit equipped with Berkovich indenter was determine values reduced Young’s modulus indentation hardness both atomic force using a diamond probe on silicon cantilever human root cementum. Three areas cementum studied: cer...

BACKGROUND/AIMS Mechanical removal of carious dentine based on perceived hardness is subjective and tends to be excessively destructive; chemo-mechanical techniques have been proposed as being more objective and conservative. The aims of the present study are to use X-ray microtomography (XMT/micro-CT) to determine the three-dimensional mineral concentration distribution in sound, carious and e...

This study compared dentine demineralization induced by in vitro and in situ models, and correlated dentine surface hardness (SH), cross-sectional hardness (CSH) and mineral content by transverse microradiography (TMR). Bovine dentine specimens (n = 15/group) were demineralized in vitro with the following: MC gel (6% carboxymethylcellulose gel and 0.1 M lactic acid, pH 5.0, 14 days); buffer I (...

OBJECTIVE The aim of this study was to evaluate the cytotoxicity of three dentine bonding agents (G-Bond, Clearfil S(3) Bond and Clearfil SE Bond X) in cell-culture perfusion. METHODS In this experiment, 8×10(4) TCPC SV40 cells (bovine-pulp-derived cells transfected with simian virus 40 large T-antigen) in MEM-alpha media, 20%FCS were seeded on mesh in a 6-well plate and incubated at 37 °C wi...

The benefits of using sodium hypochlorite (NaOCl) and ethylenediaminetetraacetic acid (EDTA) as endodontic irrigants, and calcium hydroxide as an inter-appointment medicament, are well known to dentists. Many steps undertaken during endodontic treatment and retreatment are rather mechanical in nature, and less attention is committed to understanding the biological issues underlying endodontic t...

Dental enamel is the structure which is first exposed to the attack of dental caries, and the success or failure of this attack depends on many factors, among them the chemical nature and structure of the enamel. Some authors (Pincus, 1939; Gottlieb, 1947) go so far as to postulate that the caries process is initially a proteolytic one. In this connexion the nitrogenous material of enamel is of...

introduction: dentine hypersensitivity (dh) is characterized by a short sharp pain arising from the exposed dentine, in response to a thermal, evaporative, tactile, osmotic or chemical stimulus. the aim of this study was to evaluate the efficacy of nd:yag laser therapy and desensitizing gel (sensikin®, laboratorios kin s.a., barcelona, spain) in treatment of dentine hypersensitivity. methods: a...

BACKGROUND Controlled, uniformly tapered radicular preparation is a great challenge in endodontics. Improper preparation can lead to procedural errors like transportation of foramen, uneven dentine thickness, stripping of root canal, formation of ledge, zip, and elbow in curved canals. These procedural errors and their sequel can adversely affect the prognosis of treatment. AIM/OBJECTIVES The...

OBJECTIVE To compare the effectiveness of annual topical application of silver diamine fluoride (SDF) solution, semi-annual topical application of SDF solution, and annual application of a flowable high fluoride-releasing glass ionomer in arresting active dentine caries in primary teeth. METHODS A total of 212 children, aged 3-4 years, were randomly allocated to one of three groups for treatm...

BACKGROUND Numerous cross-sectional studies of school-age children have observed that exposure to manganese (Mn) adversely affects neurodevelopment. However, few prospective studies have looked at the effects of both prenatal and postnatal Mn exposure on child neurodevelopment. METHODS We measured Mn levels in prenatal and early postnatal dentine of shed teeth and examined their association w...