background: dental pulp stem cells can be used in regenerative endodontic therapy. the aim of this study was to introduce an efficient method for dental pulp stem cells isolation. materials and methods: in this in-vitro study, 60 extracted human third molars were split and pulp tissue was extracted. dental pulp stem cells were isolated by the following three different methods: (1) digestion of ...

Non surgical root canal treatment is carried out to salvage the teeth in oral cavity. Although the success rate is found to be ranging from 81-95%, for variety of reasons failure of root canal is still seen in day to day practice. This is a cross sectional study done in Nepali population to evaluate the causes for such failures. It was found that inadequate obturation (45%) was the major cause ...

Two new Parasavaljevia species are described, one from Puerto Madryn, and one from El Límite beach, San Jorge Gulf, Chubut Province, Argentina. The two species are characterised by a combination of characters. Parasavaljevia uncinoa n. sp. is characterised by having a long rectangular cirrus, positioned at the level of the inner labial setae, rectangular mandibles with prominent lateral hooks b...

INTRODUCTION A critical step in biomaterial selection effort is the determination of material as well as the biological properties of the target tissue. Previously, the selection of biomaterials and carriers for dental pulp regeneration has been solely based on empirical experience. METHODS In this study, first, the linear viscoelastic material functions and compressive properties of miniatur...

From among age estimation procedures we chose two for comparison. One of them is the standard method based on root dentin transparency; the other one is based on coronal pulp cavity heigth. The material consisted of X-ray photographs and longitudinally sectioned teeth of 276 individuals (174 males and 102 females) of known age. The comparison shows that the poorly known TCI method is at least a...

BACKGROUND Dental pulp stem cells can be used in regenerative endodontic therapy. The aim of this study was to introduce an efficient method for dental pulp stem cells isolation. MATERIALS AND METHODS In this in-vitro study, 60 extracted human third molars were split and pulp tissue was extracted. Dental pulp stem cells were isolated by the following three different methods: (1) digestion of ...

Dental preparation sometimes causes transient congestion, edema, and necrosis of the pulp. We hypothesized that nitric oxide (NO) is involved in the pathophysiological changes in pulp after preparation. The mRNA and protein expression of the inducible isoform of NO synthase (iNOS) was examined in murine pulp after dental preparation. The effects of NO on the proliferation, mineralization, and a...

We investigated three-dimensional decrease in the volume of the pulp chamber caused by age-related secondary dentin formation using micro-CT and evaluated the applicability of the results to evaluation of age taking into account sex, age and tooth type. Decrease was slightly higher in females than in males, and a higher correlation between decrease and aging was observed in females. A compariso...

OBJECTIVE This study aimed to evaluate in vivo the occurrence of secondary caries and dentin characteristics in permanent molars after traditional amalgam restorations, by means of clinical visual examination, radiographs and laser-induced fluorescence (LF) (DIAGNOdent). METHODS Thirty first permanent molars of 30 schoolchildren in the 7 to14 year-old age group were included. Caries was remov...

Objective- Due to its specific position, the canine tooth is more exposed to the injuries, fractures and pulp necrosis. In these cases, the damaged teeth are usually pulled out. The root of this tooth is very large and sometimes extends over the premolar teeth. To extract it, a large amount of alveolar bone tissue should be harvested. Moreover, the cavity remains very large, wh...