نتایج جستجو برای: density gradient centrifugation

تعداد نتایج: 528946  

Journal: :Clinical cancer research : an official journal of the American Association for Cancer Research 2003
Megan K Baker Kaidi Mikhitarian Walid Osta Kathi Callahan Rana Hoda Frank Brescia Rayna Kneuper-Hall Michael Mitas David J Cole William E Gillanders

PURPOSE The goal of this study was to develop a molecular diagnostic assay to detect circulating breast cancer cells in the peripheral blood for the purpose of staging breast cancer. Our aim was to make available an assay that was not limited by the low concentration of circulating breast cancer cells and the background gene expression that is typically found in peripheral blood. EXPERIMENTAL...

Journal: :The Biochemical journal 1971
D Allan M J Crumpton

The degree of solubilization of pig lymphocyte plasma membrane by sodium deoxycholate was determined at a variety of temperatures and detergent concentrations. Approx. 95% of the membrane protein was soluble in 2% deoxycholate at 23 degrees C. Some of the biological activities of the membrane survived this treatment. The leucine beta-naphthylamidase activity was more readily soluble than the 5'...

Journal: :Middle East Fertility Society Journal 2022

Abstract Objective Density gradient centrifugation (DGC) is commonly used for sperm preparation before assisted reproductive technology (ART) procedures. This technique separates superior motile spermatozoa with normal morphology from the total population. However, there still controversy as to effects of this separation on cell DNA integrity which a determining element in process fertilization...

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