Cytochrome P450 (P450) 2S1 is one of the orphan P450s without a clear physiological function. Controversy has arisen as to whether it can interact with NADPH-P450 reductase and accept electrons. The reduction of 1,4-bis{[2-(dimethylamino-N-oxide)ethyl]amino}-5,8-dihydroxyanthracene-9,10-dione (AQ4N) by P450 2S1 was confirmed, and the NADPH consumption rates were measured aerobically and anaerob...

Electrochemical biosensors that combine high selectivity of biochemical affinity with precise electrochemical detection are one the most necessary and powerful tools for assessing environmental pollution. This review addresses assess pollutant toxicity. using enzyme activity inhibition, DNA, whole cells, cytochrome P450 will be introduced, their advantages applications discussed, trends challen...

The cytochrome P450 mediated oxidative metabolism of a series of para-substituted anisoles has been examined using ab initio CASSCF (complete active space self-consistent field) calculations. On the basis of these calculations, oxidative metabolites were rationalized using the concept of hydrogen atom abstraction, spin delocalization, and hydroxyl radical recombination, which is believed to gov...