Age-related changes of gene expression contribute to the physiological alteration observed with human ageing. Herein, the abundance of a selection of 148 transcripts involved in immunosenescence and stress response was compared in total RNA of PBMC of healthy young to middle-age probands (35.0 +/- 6.5 year old) and healthy old probands (82.5 +/- 6.8 year old). This study provides a list of 16 d...

Refined fuels have considerable share of pollution of marine ecosystems. Gasoline is one of the most consumed fuel worldwide, but its effects on marine benthic primary producers are poorly investigated. In this study, Ulva lactuca was chosen as a biological model due to its cosmopolitan nature and tolerance to high levels and wide range of xenobiotics and our goal was to evaluate the effects of...

The bearing of lipid solubilities and substantivities of dyes on the results of cytochemical staining reactions is briefly discussed. Solubilities of indigoid dyes in various lipids are qualitatively similar to those in symtetrachloroethane. Quantitative determinations made in this solvent indicate that, in general, indigo and its 5:5'and 6:6'-substituted derivatives are sparingly soluble, wher...

The nature of secretion products forming the pellicle of the dry-type stigma surface has been investigated in Brassica by an electron-microscopic cytochemical study. In order to determine whether the pellicle is organized like a membrane, we used a series of cytochemical methods to visualize cell membranes. Three groups of techniques presenting different degrees of specificity in terms of cell ...

The effects of several concentrations of puromycin on the nucleoproteins of HeLa cells grown in monolayers were studied by cytochemical and biochemical techniques. The earliest change at all concentrations of puromycin was a decrease in a granular form of ribonucleoprotein (RNP) that is demonstrable in the normal HeLa cell by the toluidine blue-molybdate (TBM) stain. The other types of RNP reve...

Hepatic glucokinase (GK) is acutely regulated by binding to its nuclear-anchored regulatory protein (GKRP). Although GK release by GKRP is tightly coupled to the rate of glycogen synthesis, the nature of this association is obscure. To gain insight into this coupling mechanism under physiological stimulating conditions in primary rat hepatocytes, we analyzed the subcellular distribution of GK a...

Although various tissue macrophages possess high glucose-6-phosphate dehydrogenase (G6PD) activity, which is reported to be closely associated with their phagocytotic/bactericidal function, the fine subcellular localization of this enzyme in liver resident macrophages (Kupffer cells) has not been determined. We have investigated the subcellular localization of G6PD in Kupffer cells in rat liver...

Tartrate-resistant acid phosphatase (TRAcP) has been an indispensible marker for hairy cell eukemia (HCL) for over two decades. However, the traditional TRAcP cytochemical stain cannot be performed effectively on sections of paraffin-embedded tissues that are important resources for histopathologic evaluation in diagnosis and treatment of HCL. Wide variation in expression of TRAcP activity by h...

Tartrate-resistant acid phosphatase (TRAcP) has been an indispensible marker for hairy cell eukemia (HCL) for over two decades. However, the traditional TRAcP cytochemical stain cannot be performed effectively on sections of paraffin-embedded tissues that are important resources for histopathologic evaluation in diagnosis and treatment of HCL. Wide variation in expression of TRAcP activity by h...

A 14-year-old female domestic shorthair cat was presented to Tehran University Veterinary Teaching Hospital for a persistent fever, anorexia, intermittent vomiting, weight loss and weakness. The main clinical signs were pale mucous membranes, dehydration and splenomegaly. The complete blood count and serum biochemistry tests revealed non-regenerative anaemia, thrombocytopenia and increased alka...