Enzymes are useful tools as specific analytical reagents. Two different analysis methods were developed for use in the separate fields of protein science and organic synthesis. Both methods rely on the substrate specificity of enzymes. Enzyme catalysis and substrate specificity is described and put in context with each of the two developed methods. In paper I a method for C-terminal peptide seq...

A selective, sensitive, rapid and simple-handling analytical method for the determination of cyanide at low detection limits in surface and underground water, soil and industrial waste samples was developed. The method is based on a reaction, proposed by Guilbault and Kramer, where free cyanide reacts with p-nitrobenzaldehyde to form an intermediate cyanohydrin, which reacts with o-dinitrobenze...

Enantiopure α-hydroxy acids (AHAs) are versatile building blocks in organic synthesis. They are used for obtaining pharmaceutically and biologically active substances, such as vitamins. α-Hydroxy acids are easily synthesized from cyanohydrins. Thus, molecules with cyanohydrin substructures are interesting intermediates for the synthesis of AHAs. Usually, cyanohydrins are prepared by the additio...

understanding the performance and role of each formation in a petroleum play is crucial for the efficient and precise exploration and exploitation of trapped hydrocarbons in a sedimentary basin. the lorestan basin is one of the most important hydrocarbon basins of iran, and it includes various oil-prone potential source rocks and reservoir rocks. previous geochemical studies of the basin were n...

Bonavita (1) found that the reaction of pyridoxal (PAL) and of pyridoxal 5•Œ-phosphate (PAL-P) with cyanide in a slightly alkaline solution produced highly fluorescent compounds and this reaction has been applied to fluoro metric determination of PAL and PAL-P as "cyanohydrin method" . Recently it was found that PAL and PAL-P were oxidized with oxygen by a catalytic action of cyanide to 4pyrido...

A m ethod is prese nted for preparing C'4-l abeled L-ascorbi c acid (vi tamin C) from labeled banum D-gluconate or D-glucose. The process is adapted from t he commercial synthesis of vi tamin C t hrough sorbitol, L-sorbose and potassium di-O-isopropylidene-2kelo-L-guloll.ate. In .th e cyanohydrin . synt hesis of labeled D-glucose, barium D-gluconate is used as an lI1term ed~at~ . Fo: prepanng l...

The methyl linamarin that occurs naturally in linen flax (Zinum usifafissimum L.) seedlings was shown to have the R configuration, i.e. it is identical with (R)-lotaustralin, (R)-2hydroxy-Z-methylbutyronitrile-P -D glucopyranoside. However, when a mixture of the two enantiomers (R,S)-2-hydroxy-2-methylbutyronitrile, was administered to excised flax shoots, both (R)-lotaustrahn and its epimer (S...

The meso-salicylaldehyde substituted BODIPY was synthesized over a sequence of steps and characterized by X-ray crystallography, mass, NMR, absorption, fluorescence and electrochemical techniques. The crystal structure showed the presence of strong intramolecular hydrogen bonding between hydroxyl and formyl groups, which induces rigidity in the BODIPY core and makes the BODIPY relatively more f...