The expression pattern of pluripotency markers in adipose tissue-derived stem cells (ADSCs) is a subject of controversy. Moreover, there is no data about the signaling molecules that regulate these markers in ADSCs. In the present study, we studied the roles of leukemia inhibitory factor (LIF) and miR-302 in this regard. Freshly isolated mouse ADSCs expressed hematopoietic, mesenchymal, and plu...

The tribe Diglottini Eichelbaum, 1909 comprises two halophilous rove beetle genera Diglotta Champion, 1899, and Paradiglotta Ashe & Ahn, 2004. The tribe contains eight known species distributed in the Nearctic and West-Palaearctic regions, and also Fiji Islands and New Zealand. This tribe is recorded for the first time from South America with the description of a new species, Diglotta brasilien...

Background: NADPH:cytochrome P450 oxidoreductase (POR) catalyses one-electron reduction of hypoxia-activated prodrugs (HAP). Results: Knockout of POR in tumour cells inhibited activation of some HAP (e.g. 5nitroquinoline SN24349) but not others (e.g. PR104A, TH-302). Conclusion: POR at endogenous levels plays a variable role in HAP activation. Significance: POR expression alone is not a suffici...

PURPOSE Chemotherapy targets rapidly proliferating tumor cells, but spares slowly proliferating hypoxic cells. We hypothesized that nutrition of hypoxic cells would improve in intervals between chemotherapy, and that hypoxic cells destined to die without treatment would survive and proliferate. EXPERIMENTAL DESIGN We therefore evaluated repopulation and reoxygenation following chemotherapy, a...

Sheep liver cytoplasmic aldehyde dehydrogenase was labelled by reaction with the substrate p-nitrophenyl di[14C]methylcarbamate. After tryptic digestion and peptide fractionation the labelled residue was identified as Cys-302. This is the first unequivocal identification of the essential enzymic nucleophile in the esterase activity of aldehyde dehydrogenase. By implication, Cys-302 is probably ...

This preliminary study aims to obtain the lithium compounds from a geothermal brine through solvent extraction. The samples were collected PT Geodipa Energi Geothermal Power Plant in Dieng, Central Java, Indonesia. ICP analysis was conducted measure chemical composition. Brine’s content 62.73 ppm with dominant impurities silica (Si), calcium (Ca), potassium (K), sodium (Na), and boron (B). Sili...

Single agent doxorubin was introduced for the treatment of soft tissue sarcoma beginning in the 1970’s. Since then, combination therapies with an anthracylcine backbone are commonly used, but have failed to show significant improvements in survival compared to single agent doxorubicin. TH-302, a pro-drug activated by tumor hypoxia, is a promising new therapeutic agent for soft tissue sarcomas. ...