In recent years, substantial advances have been made in the development of artificial metalloenzymes. These hybrid bioinorganic catalysts seen their selectivity improved through techniques including site-directed and site-saturation mutagenesis directed evolution. Their scope has expanded by introduction various abiological metal catalysts, new anchoring strategies designs to enhance reusabilit...

Enantioselective catalysis is one of the most efficient ways to synthesize high-added-value enantiomerically pure organic compounds. As the subtle details which govern enantioselection cannot be reliably predicted or computed, catalysis relies more and more on a combinatorial approach. Biocatalysis offers an attractive, and often complementary, alternative for the synthesis of enantiopure produ...

Subterranean clover (Trifolium subterraneum L. cv Seaton Park) was grown in solution cultures containing adequate nitrogen both with and without Cu. After Cu deficiency had developed, Cu(2+) was added to some deficient plants and Cu content, protein content, and activities of three Cu metalloenzymes (diamine oxidase [EC1.4.3.6], ascorbate oxidase [EC1.10.3.3] and o-diphenol oxidase [EC1.10.3.1]...

In this study, the possible improvement of antibacterial and washing resistance performance 100% cotton fabrics were investigated.The purpose research was to evaluate 1,2,3,4-butantetracarboxylic acid (BTCA), citric monohydrate (CA) sodium hypophosphite (SHP) as an crosslinking agent for textile substrates against, Staphylococcus aureus (ATCC 43300), Bacillus subtilis (NRRL NRS-744), Escherichi...

Routes to pincer and chelate carbene complexes of Pd, Rh, and Ir include double geminal CH activation, metallation, and CH activation. Abnormal binding via imidazole C5 can occur, and ion pairing can strongly influence C2 vs. C5 binding. Prior ligand binding via pyridine N before metallation is not essential. Computational methods are used extensively, for example, to compare N vs. C binding in...

The assembly formation of Zn cluster compounds comprising pyridinealcohol ligands is described and their solution and solid state features have been determined using NMR spectroscopy and X-ray diffraction. The di-Zn complex 5 and tetra-Zn complex 7 may serve as accessible structural models for the active site of various multinuclear Zn-containing metalloenzymes. Their phosphoester cleavage acti...

In recent years, significant progress has been made in the computational design of functional artificial enzymes. When combined with directed evolution protocols, their efficiencies are comparable to those obtained with catalytic antibodies. In comparison, the in silico creation of artificial metalloenzymes remains challenging. This could be due to the difficulty in computing both the transitio...

Zinc is present in most body cells of human beings, but its concentration is very low. Zinc(II) ion has been widely found in several metalloenzymes such as zinc-peptidases, human carbonic anhydrase and alkalinephosphatase [1]. Today more than 200 different zinc proteins are known which include numerous essential enzymes [2]. Metallothioneins, proteins containing zinc, remain unique in character...