BACKGROUND Precise spatial control and patterning of cells is an important area of research with numerous applications in tissue engineering, as well as advancing an understanding of fundamental cellular processes. Poly (dimethyl siloxane) (PDMS) has long been used as a flexible, biocompatible substrate for cell culture with tunable mechanical characteristics. However, fabrication of suitable p...

S cientists have been fascinated for decades by the ability to drive differentiation of pluripotent or multipotent stem cells down Waddington's epigenetic landscape — a conceptual framework that explains how genes interact with their surroundings to produce a cell's phenotype. Major efforts have identified a number of soluble biochemical factors that control stem cell plasticity and differentia...

The development of xeno-free, chemically defined stem cell culture systems has been a primary focus in the field of regenerative medicine to enhance the clinical application of pluripotent stem cells (PSCs). In this regard, various electrospun substrates with diverse physiochemical properties were synthesized utilizing various polymer precursors and surface treatments. Human induced pluripotent...

BACKGROUND The pluripotency and self renewing properties of human embryonic stem cells (hESC) make them a valuable tool in the fields of developmental biology, pharmacology and regenerative medicine. Therefore, there exists immense interest in devising strategies for hESC propagation and differentiation. Methods involving simulation of the native stem cell microenvironment, both chemical and ph...

Polymeric biomaterials are used as substitutes for damaged tissue and for the stimulation of tissue regeneration. One class of polymeric biomaterials are bioresorbable polymers that degrade both in vitro and in vivo and are used as a temporary support for tissue regeneration. Among the various types of bioresorbable polymers, -hydroxy acids including the different forms of poly(lactic acid) (P...

The physico-chemical properties of serpentine soils lead to strong selection of plant species. Whereas many studies have described the serpentine flora, little information is available on the fungal communities dwelling in these sites. Asbestos minerals, often associated with serpentine rocks, can be weathered by serpentine-isolated fungi, suggesting an adaptation to this substrate. In this stu...

glycolytic and capable of producing lactic acid from substrates other than glucose; they showed no metabolic response to the presence of insulin in the medium. Fewer Novikoff cells than Reuber cells remained viable after 10 days in culture. It is suggested that the inability of the hepatoma cells to survive in filter well culture may be a reflection of the extent to which metabolism has become ...

from OXPHOS to glycolysis [6]. The pluripotent marker OCT4 affects several target genes (e.g. ATP5D) associated with energy metabolism which are in favor of glycolysis [1]. Various new hPSC culture systems have been developed in recent years, including defined serum-free media (e.g., mTESR, StemPro, and E8) and synthetic substrates (e.g., synthemax) [7,8]. How the subtle differences in these cu...

Much work is needed in the design of more effective bone tissue engineering materials to induce the growth of normal bone tissue. Nanotechnology offers exciting alternatives to traditional bone implants since bone itself is a nanostructured material composed of nanofibered hydroxyapatite welldispersed in a mostly collagen matrix. For this purpose, poly-lactic-co-glycolic acid (PLGA) was dissolv...

ABSTRACT Microstructured Photoresist (PR) has been applied for cell culture substrate, but their effects on cell behaviors are still unclear. Here, we evaluated the effects of PR substrates on human induced pluripotent stem cells (hiPSCs), which have a great potential for clinical applications. By comparing two types of epoxy based PRs with and without antimony salt, both PRs have affected on h...