We compared group A Streptococcus (GAS) culture with a rapid helicase-dependent amplification (HDA) method using 1,082 throat swab specimens. The HDA method demonstrated 98.2% sensitivity and 97.2% specificity. GAS prevalence by culture was 20.7%, and it was 22.6% using the HDA method. In 35 min, the HDA method provided rapid, sensitive GAS detection, making culture confirmation unnecessary.

staphylococcus spp., is a common bacteria in cattle mastitis. detection of the low number of bacteria requires a sensitive method. we used multiplex polymerase chain reaction (m-pcr) assay to detect the genes encoding sea, seb, sec toxins, that are specific in s. aureus bacteria. the gene of 23s rrna that is conserved in all staphylococcus spp., also was used. due to the stiffness and low effec...

Mycoplasma synoviae (M. synoviae) is a major worldwide poultry pathogen that causes serious economic losses in the poultry industry. This study was designed to detect M. synoviae through culture isolation and polymerase chain reaction (PCR) assay to demonstrated the involvement of M. synoviae infection in trachea and the lung/air sac samples taken from commercial broiler chicken farms in 3 m...

The definitive diagnosis of spontaneous bacterial peritonitis is made by a positive ascitic fluid culture. Causative organisms cannot be isolated in up to 65% of patients with well-defined spontaneous bacterial peritonitis, probably due to inadequate ascites culture techniques. We prospectively compared two ascites culture methods: conventional (on chocolate agar and thioglycolate broth) and mo...

the development of the dominant lactobacilli population during traditional liqvan cheese production was investigated using two different molecular methods of: culture dependent rapd vs culture independent dgge. either one of the techniques showed a high degree of capability to identify and detect of this group of lactic acid bacteria. however, some differences were conspicuous during the detect...

BACKGROUND/AIMS The aim of this study was to evaluate the peritonitis-causing bacteria detected in peritoneal fluid using a blood culture bottle in patients undergoing continuous ambulatory peritoneal dialysis (CAPD). METHODS One-hundred and eleven dialysates from 43 patients suspected of peritonitis related to CAPD were retrospectively evaluated between May 2000 and February 2008. In all cas...

introduction: awareness of the species of shigella has particular importance in the way of dealing with an outbreak, controlling it, and treating patients. the purpose of this study was to use the multiplex pcr method and comparison with the culture method to detect shigella species, as well as analysing the antibiotic resistance pattern in its different species. methods: simultaneously, the fe...

the purpose of this article is to examine the relationship between organizational culture and leadership styles. organizational culture is important factor that can be influence organizational performance and also leadership is one of the key parts that can be affected organizational success. however, recognition about the relationship between these two effective factors on organizational perfo...

Current techniques of in vitro cell cultures are able to mimic the in vivo environment only to a limited extent, as they enable cells to grow only in two dimensions. Therefore cell culture approaches should rely on scaffolds that provide support comparable to the extracellular matrix. Here we demonstrate the advantages of novel nanostructured three-dimensional grids fabricated using electro-spi...