The existence of a method for culturing porcine muscle cells would greatly facilitate the development of new breeding criteria for stress resistance and growth regulation in pig breeding. Also, many effects of nutritional or pharmacological components that influence animal performance could be studied first in muscle cell preparations. Therefore, we developed a specific procedure to culture por...

OBJECTIVES The fibrocytes, the mesenchymal cells and the colony forming units are included in the concept fibroblast-like, peripheral-blood circulating stem cells. The aim of this work was to establish the culture conditions. MATERIAL AND METHOD Peripheral blood mononuclear cells were isolated from 25 samples collected from the cephalic vein. These were cultured in McCoy's 5A enriched with 20...

OBJECTIVES To provide an integrative review of the existing literature on the interrelationships among sleep, culture, and medical conditions in children. METHODS A comprehensive literature search was conducted using PubMed, Medline, and PsychINFO computerized databases and bibliographies of relevant articles. RESULTS Children with chronic illnesses experience more sleep problems than healt...

Cystoderma amianthinum, one of edible fungi belongs to Agaricaceae of Basidiomycota, has a good taste and flavor. This study was carried out to obtain the basic informations for the optimum mycelial growth of C. amianthinum. The optimal conditions for the mycelial growth were 25℃ and pH 5 in potato dextrose agar (PDA). C. amianthinum showed the favorable growth in the PDA and yeast malt extract...

The potential clinical utility of engineered muscle is currently restricted by limited in vitro capacity of expanded muscle precursor cells to fuse and form mature myofibers. The purpose of this study was to use isotropic skeletal muscle sheets to explore the impact of (1) fibroblast coculture and (2) fibroblast-conditioned media (fCM) on in vitro myogenesis. Muscle sheets were prepared by seed...

Organic gelling agents such as agar and agarose provide a heterotrophic substrate for growth of illuminated tobacco callus. When green cells are incubated in CO(2)-free air on a medium lacking sucrose but solidified with 1% agar, an increase in relative dry weight is sustained through two passages. Similar results with different inoculum sources, and with three brands of agar and two forms of a...

Dear Readers, Imagine yourselves at the outer space, the void that lies beyond the uppermost reaches of the earth's atmosphere, comprising all other objects in the universe 7. Although it is a void, outer space may be thought of as an environment, where unprotected humans would perish. The principal environmental characteristic of outer space is the vacuum, where gas molecules are practically n...

Selenium (Se) as a vital trace element has many biological activities such as anti-inflammation and anti-oxidation. Selenomethionine as an organic selenium plays a vital role in the response to oxidative stress. At present, Saccharomyces cerevisiae is one of the best microorganisms that has the ability to accumulate selenium. Production of Seleno-yeast was done by growing Saccharomyces cerevisi...

Selenium (Se) as a vital trace element has many biological activities such as anti-inflammation and anti-oxidation. Selenomethionine as an organic selenium plays a vital role in the response to oxidative stress. At present, Saccharomyces cerevisiae is one of the best microorganisms that has the ability to accumulate selenium. Production of Seleno-yeast was done by growing Saccharomyces cerevisi...