The growth of microorganisms in a synthetic medium is influenced by a number of physical and chemical factors. The nutrient composition of a culture medium plays a most important role in microbial growth (Tortora and Funk, 1995). Fungi grow on diverse habitats in nature and are cosmopolitan in distribution requiring several specific elements for growth and reproduction (Singh and Sharma, 2014)....

In order to increase the mycelial production of Phellinus linteus, which exhibits potent anticancer activity, some ingredients of the medium used to culture P. linteus were investigated. The optimal medium composition for the production of Phellinus linteus was determined to be as follows: fructose, 40 g/l; yeast extract, 20 g/l; K2HPO4, 0.46 g/l; KH2PO4, 1.00 g/l; MgSO4·7H2O, 0.50 g/l; FeCl2·6...

Bacterial infections, most commonly spontaneous bacterial peritonitis in patients with ascites, occur in one third of admitted patients with cirrhosis, and account for a 4-fold increase in mortality. Bacteria are isolated from less than 40% of ascites infections by culture, necessitating empirical antibiotic treatment, but culture-independent studies suggest bacteria are commonly present, even ...

When cultures of bacteria are transferred in the usual way, both the character of the cells, and the composition of the medium are constantly changing. If the cell concentration is held constant, on the other hand, the composition of the medium (including enzymes and viruses) in which the growth occurs, must also be constant and any change in the concentration of any substance indicates a chang...

BACKGROUND recent reports have suggested the applicability of salivary amino acids as markers for various diseases. In order to understand the role of macrophages in the age-related changes of salivary amino acid composition, we compared the amino acid production and consumption between control and activated macrophages. MATERIALS AND METHODS mouse macrophage-like cells (RAW264.7, J774.1) wer...

Accepted: December 10, 2004. SUMMARY Venous leg ulcers are an important cause of morbidity in a significant percentage of the world population. The percentage of leg ulceration, either active or healed, in the European population is about 1%-2%. The aim of this study was to analyze the rate of colonization and qualitative composition of the bacterial flora isolated from leg ulcers in patients a...

Background Intra-abdominal abscesses are localized collections of pus, which generally arise from a breach in the normal mucosal defense barrier that allows bacteria from gastrointestinal tract, and less commonly from the gynecologic or urinary tract, to induce inflammation, resulting in an infection. The microbiology of these abscesses is usually polymicrobial, associated with the primary dise...

Sputum cultures are an important tool in monitoring the response to tuberculosis treatment, especially in multidrug-resistant tuberculosis. There has, however, been little study of the effect of treatment regimen composition on culture conversion. Well-designed clinical trials of new anti-tuberculosis drugs require this information to establish optimized background regimens for comparison. We c...

In a series of continuous culture experiments involving N-limited growth of the chrysophyte Pavlova lutheri (Mono) I found that large deviations in cellular C : N : P ratios from the Redfield ratio of 106 : 16 : 1 occurred only as a function of low relative growth rate and not of low light intensity. By contrast, light-limited phy-toplankton in continuous culture can attain the Redfield chemica...

We have studied the autotrophic growth of the marine microalga, Isochrysis galbana Parke, in a batch photobioreactor, comparing five different culture media and analysing the influence of each on growth kinetics as well as on the fatty-acid composition and protein content of the biomass. All the experiments were performed at 15 degrees C, with the culture medium at pH 8.0, a specific rate of ai...