nano fillers are part of new studies to enhance various properties of fluids and solids. for example, adding nanoparticles as an enforcement in nanocomposites or as an additive to improve thermal or electrical properties in nano-fluids are of extreme importance in science and industry. there are numerous methods to uniformly disperse nanoparticles in fluid and solid phase. one of the well-used ...

Size-quantized CdS nanocrystals of controlled dimensions are synthesized within monodisperse egg lecithin vesicles made by detergent depletion. Cryo-transmission electron microscopy (cryo-TEM) enables imaging in situ both of the vesicle wall and of the encapsulated nanocrystal. Cryo-TEM images confirm that only one particle per vesicle forms and that nanocrystal diameter is determined by the nu...

Transmission electron microscopy (EM) is a versatile technique that can be used to image biological specimens ranging from intact eukaryotic cells to individual proteins >150kDa. There are several strategies for preparing samples for imaging by EM, including negative staining and cryogenic freezing. In the last few years, cryo-EM has undergone a 'resolution revolution', owing to both advances i...

The development of a microfluidically cryo-cooled and inductively coupled spiral microcoil for magnetic resonance (MR) microscopy with high signal-to-noise ratio (SNR) is presented. The microcoil was inductively coupled to a secondary coil that was directly coupled to a transmission line. This configuration eliminated thermal flux through direct connection when the microcoil was cryo-cooled to ...

Until only a few years ago, single-particle electron cryo-microscopy (cryo-EM) was usually not the first choice for many structural biologists due to its limited resolution in the range of nanometer to subnanometer. Now, this method rivals X-ray crystallography in terms of resolution and can be used to determine atomic structures of macromolecules that are either refractory to crystallization o...

The cryo scanning electron microscopy (cryo-SEM) as well as high-pressure-freezing (HPF) belongs to cutting edge techniques in electron microscopy of hydrated samples [1]. However, their combination is not always easily applicable. Here, we present a way of combining high-pressure-freezing using EM PACT2 (Leica Microsystems) that fixes hydrated samples on a 1.4 mm sapphire discs and high resolu...

Cryo-electron microscopy (cryo-EM) is an emerging experimental method to characterize the structure of large biomolecular assemblies. Single particle cryo-EM records 2D images (so-called micrographs) of projections of the three-dimensional particle, which need to be processed to obtain the threedimensional reconstruction. A crucial step in the reconstruction process is particle picking which in...

Single-particle cryo-electron microscopy (cryo-EM) has become one of the mainstream structural biology techniques because its ability to determine high-resolution structures dynamic bio-molecules. However, cryo-EM data acquisition remains expensive and labor-intensive, requiring substantial expertise. Structural biologists need a more efficient objective method collect best in limited time fram...