Saturation mutagenesis of the 2,4-dinitrotoluene dioxygenase (DDO) of Burkholderia cepacia R34 at position valine 350 of the DntAc alpha-subunit generated mutant V350F with significantly increased activity towards o-nitrophenol (47 times), m-nitrophenol (34 times), and o-methoxyphenol (174 times) as well as an expanded substrate range that now includes m-methoxyphenol, o-cresol, and m-cresol (w...

Two patients exposed to high concentrations of toluene in air (greater than 7000 mg/m3) were found at the bottom of a small swimming pool under construction. Their symptoms were stupefaction, paresis, and amnesia. Patient A had been exposed for three hours and patient B for two hours. Ninety minutes after the exposure, the toluene blood concentration in patient A was 4.1 mg/l and in patient B 2...

1. Either phenol red or brom-cresol purple may be used as indicators in the preparation of lactose agar plates for the isolation of members of the typhoid-dysentery group of bacteria from stools. Of the two, brom-cresol purple gives sharper differentiation and is to be preferred. 2. These indicators exercise no restraining influence upon the growth of cultures of the typhoid bacillus or paratyp...

Development of coal gasification plants in the United States for the generation of electrical power (SPENCER et al. 1982) may increase the probability of accidental spills of coal-derived phenols and monohydric cresol isomers into streams and rivers. Although Europe has experienced several accidental spills from coal fired electrical plants and phenol producing plants (ALABASTER et al. 1973), m...

.............................................................................................12 Introduction..........................................................................................13 Cues of larval food.................................................................................16 Cues of mosquito immature stages................................................................

The disinfectant effects (DEs) of 10 types of chemicals, defined by their ability to destroy or inhibit oocysts and consequently prevent sporulation of Eimeria tenella field isolate, were evaluated in vitro. Correct species assignments and sample purities were confirmed by the singular internal transcribed spacer (ITS)-PCR analysis. A total of 18 treatments were performed, and the disinfection ...

In two separate experiments 10 healthy men each were exposed at rest in an exposure chamber to about 200 ppm toluene in the air. Hippuric acid, o-, m-, p-cresol, and phenol in urine were detected by capillary gas chromatography at the beginning and at the end of exposure, and at variable times after the cessation of exposure. In addition toluene in blood was determined at the same intervals. Th...

Abstract Crude phenols extracted using organic solvent from the wastewater of a typical fixed-bed gasification process was used as raw material, and distillation range analyzed. The wide narrow fractions material derived analysis were cut real boiling point device. phenolic compounds in different then qualitatively quantitatively analyzed by gas chromatography after derivatization pretreatment....

Tricresyl phosphate (TCP) forms protective films on moving mechanical components through thermally driven decomposition and interactions with the ferrous surfaces of components. These reactions are hidden from view in interfaces, but known to be sensitive both surface material isomeric form TCP. Here, temperature-programmed reaction spectroscopy (TPRS) gas chromatography-mass (GC-MS) complement...