Creatine kinase isoenzyme BB from rat brain was incubated with serum, dialyzed serum, or plasma, with added carboxylic acids, thiols, buffers, or cations. It was found to be very unstable at 37 degrees C under all these conditions, being transformed to CK-BB', a form that migrates like CK-MB on agarose electrophoretic plates. This transformation is enhanced at alkaline pH and, especially, by Zn...

Carvacrol (CAR), a naturally occurring phenolic monoterpene, has been shown to possess diverse biological activities. The present study was undertaken to evaluate the cardioprotective potential of CAR against myocardial ischemic damage in a rat model of acute myocardial infarction. CAR significantly diminished the infarct size and myocardial enzymes including creatine kinase (CK), the MB isoenz...

Certain types ofmacro-CK, type 1, have been reported to cause false increases in CK-MB by co-migration on electrophoresis (1-3). I report two such cases in which the macroCK was an IgA with anti-CK-BB specificity. According to methods proposed by Medeiros et al. (1), immunoprecipitation and subsequent analysis by elecrophoresis was used to separate interfering macre-CK from CK-MB. CK electropho...

Physiological changes, and subsequent sleep responses, were recorded in a male subject during and following 338 miles of continuous walking and consequent sleep deprivation. One hundred and thirty hours of walking and a seventy-two hours post-walk recovery period were monitored. The subject walked at approximately 55% of maximum oxygen uptake (VO2 max), heart rate ranged between 102-106 b/min, ...

Muscle symptoms and signs are a frequent reason for general neurological consultations. Weakness is the most reliable clinical indicator of myopathy. Fatigue and exercise intolerance and myalgias frequently occur in non-myopathic conditions. Cramps and myoglobinuria are more often due to systemic factors than being a sign of a metabolic or other myopathy. Contractures and myotonia are rare find...

AIM To evaluate the usefulness of the serum creatine kinase: aspartate aminotransferase (CK:AST) ratio in differentiating between myocardial and non-myocardial increases in serum creatine kinase activity in the elderly. METHODS A three month prospective study of all patients admitted to an acute geriatric unit who were clinically assessed and investigated with electrocardiograms and measureme...

We assessed the changes in sensitivity and specificity of creatine kinase (CK) isoenzymes at various times after acute myocardial infarction. CK-B was measured by an immunoinhibition technique. CK-MB was measured both by column chromatography on Sephadex DEAE A-50 and by electrophoresis on agarose gel. For CK-B, the sensitivity in detecting infarct varied from 11% in the first 8 h after onset o...

Free radicals of X-ray-induced water radiolysis, either directly or indirectly via their reaction products, reduce the activity of both dimeric cytoplasmic muscle-type creatine kinase (MM-CK) and octameric mitochondrial creatine kinase (Mi-CK) to virtually zero. Similarly values of the characteristic D(37)-dose of enzyme inactivation (dose required to reduce enzyme activity to 37%) were found f...

To examine the effects of a prerace whole-body cold shower on muscle soreness (MS) and on serum creatine kinase (CK) and creatine kinase MB (CK-MB) isoenzyme activities, 16 experienced distance runners were randomly assigned to one of two treatment categories prior to running a marathon: cold shower (n = 8) or without cold shower (n = 8). Venous blood samples were drawn 3 days before the race, ...