objective(s): fluvoxamine is a well-known selective serotonin reuptake inhibitor (ssri); despite its anti-inflammatory effect, little is known about the precise mechanisms involved. in our previous work, we found that ip administration of fluvoxamine produced a noticeable anti-inflammatory effect in carrageenan-induced paw edema in rats. in this study, we aimed to evaluate the effect of fluvoxa...

We selected for increased phenotypic expression of a synthetic cox2::arg8m-G66S reporter gene inserted into Saccharomyces cerevisiae mtDNA in place of COX2. Recessive mutations in ras2 and cyr1, as well as elevated dosage of PDE2, allowed cox2::arg8m-G66S to support Arg prototrophy. Each of these genetic alterations should decrease cellular cAMP levels. The resulting signal was transduced throu...

In this first comparative in vitro study, linoleyl hydroxamic acid (LHA), a simple and stable derivative of linoleic acid, was tested as an inhibitor of several enzymes involved in arachidonic acid metabolism in mammals. The tested enzymes were human recombinant 5-lipoxygenase (h5-LO), porcine leukocyte 12-LO, rabbit reticulocyte 15-LO, ovine cyclooxygenases 1/2 (COX1/COX2), and human microsoma...

Early inhibition of inflammation suppresses the carcinogenic process. Aspirin is the most commonly used non-steroid anti-inflammatory drugs (NSAIDs), and it irreversibly inhibits cyclooxygenase-1 and -2 (COX1, COX2). Multiple randomized clinical trials have demonstrated that aspirin offers substantial protection from colon cancer mortality. The lower aspirin doses causing only minimal gastroint...

BACKGROUND Arsenic compounds have modest cytotoxic activity in solid tumors. We investigated if arsenic sulfide (As4S4) in combination with other distinct agents could enhance its cytotoxic activity. METHODS We used gastric and colon cancer cell lines to study the synergistic effect of As4S4 in combination with BRD4 inhibitor JQ1, or with chemotherapy drug cisplatin and irinotecan or with COX...

Heterocyst development was analyzed in mutants of the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120 bearing inactivated cox2 and/or cox3 genes, encoding heterocyst-specific terminal respiratory oxidases. At the morphological level, the cox2 cox3 double mutant (strain CSAV141) was impaired in membrane reorganization involving the so-called honeycomb system that in the wild-type ...

Mechanical stimulation can prevent adipogenic and improve osteogenic lineage allocation of mesenchymal stem cells (MSC), an effect associated with the preservation of -catenin levels. We asked whether mechanical up-regulation of -catenin was critical to reduction in adipogenesis as well as other mechanical events inducing alternate MSC lineage selection. In MSC cultured under strong adipogenic ...

A novel cytoplasmic male sterility (CMS) in Brassica napus (inap CMS) was selected from the somatic hybrid with Isatis indigotica (Chinese woad) by recurrent backcrossing. The male sterility was caused by the conversion of tetradynamous stamens into carpelloid structures with stigmatoid tissues at their tips and ovule-like tissues in the margins, and the two shorter stamens into filaments witho...

Dieldrin and DDE are environmental metabolites of the organochlorine pesticides aldrin DDT, respectively. During pregnancy, these chemicals can quickly infiltrate through placental barrier, accumulate in amniotic fluid fetus, act as endocrine disruptors (EDs). The aim this study was to investigate effect dieldrin their parental substances at concentrations 1 10 ng/ml on secretion PGE2 PGF2α fro...

We have used mutational and revertant analysis to study the elements of the 54nucleotide COX2 5’untranslated leader involved in translation initiation in yeast mitochondria and in activation by the COX2 translational activator, Pet1 1 lp. We generated a collection of mutants with substitutions spanning the entire COX2 5’-UTL by in vitro mutagenesis followed by mitochondrial transformation and g...