Skin cancer is one of the most common cancers. Melanoma accounts for less than 2% of skin cancer cases but causes a large majority of skin cancer deaths. Early detection of malignant melanoma remains the key factor in saving lives. However, the melanoma diagnosis is still clinically challenging. Here, we developed a confocal photothermal microscope for noninvasive, label-free, three-dimensional...

Toru Hyodo, MD, PhD, Department of Urology and Anatomy, Faculty of Medicine, Tottori University, 36-1 Nishi-Machi, Yonago 683 (Japan) Dear Sir, Birch and Fairley [1, 2] and Birch et al. [3] described glomerular bleeding giving rise to a wide range of morphological alterations in red cells, including dysmorphic red cells. They also reported that phase contrast microscopy was clinically useful fo...

Distinguishing between benign and malignant neoplasms of the skin is a daily challenge to dermatologists. With the use of a dermatoscope and other imaging devices, the diagnosis is often more precise. The confocal microscope is a device that uses a near-infrared laser to perform noninvasive imaging of the skin. The benefit is that the images immediately provide additional, cellular-level inform...

Most confocal microscopes do not produce images in real time with nonlaser light sources. The tandem scanning confocal microscope does produce such images but, because the pinhole apertures of the Nipkov disk must be placed far apart to reduce cross talk between neighboring pinholes, only 1% or less of the light available for imaging is used. We show that, by using aperture correlation techniqu...

Instead of forming an entire image at one instant, a scanning optical microscope (SOM) scans a beam of light across specimen in a regular pattern, or raster, and forms its image point by point. A point of light is focused to a diffraction-limited spot on the sample by the objective lens. An image is built up point by point by a detector, either below the slide (if the microscope is exclusively ...

Laser scanning confocal microscopy has become an invaluable tool for a wide range of investigations in the biological and medical sciences for imaging thin optical sections in living and fixed specimens ranging in thickness up to 100 micrometers. Modern instruments are equipped with 3-5 laser systems controlled by high-speed acousto-optic tunable filters (AOTFs), which allow very precise regula...