The hyper-constrained nucleoside, methylene-bridged hexopyranosyl nucleoside (BHNA) was incorporated into the antisnese oligonucleotides (AON), which show more preference for binding toward the complementary RNA (Tm loss by ca 5°C) than that with the complementary DNA (Tm loss by 10°C), vis-à-vis corresponding native duplex. The origin of reduction of Tm of the duplexes formed by the BHNA incor...

The Xenopus oocyte is a widely used system for protein expression. Investigators have had the choice between two different techniques: injection into the cytoplasm of in vitro transcribed complementary RNA (cRNA) or injection into the nucleus of complementary DNA (cDNA). We report on a third expression technique that is based on the combined injection of cDNA and purified T7 RNA polymerase dire...

The ribonucleic acid product formed by ribonucleic acid polymerase resembles its deoxyribonucleic acid template in base composition and in base sequence (l-6). The reaction in vitro results in the synthesis of RNA chains which are complementary to both DNA strands (6). Evidence for this originates from the formation of highly ordered RNA structures when the RNA polymerase product is annealed wi...

In this paper we study a generalization of the classical notions of bordered and unbordered words, motivated by DNA computing. DNA strands can be viewed as finite strings over the alphabet {A, G, C, T}, and are used in DNA computing to encode information. Due to the fact that A is Watson-Crick complementary to T and G to C, DNA single strands that are Watson-Crick complementary can bind to each...