Hepatitis B virus (HBV) infection is of global public health concern. Among various serological tests used for the diagnosis and screening of HBV infection, the enzyme-linked immunosorbent assay (ELISA) to detect hepatitis B surface antigen (HbsAg) is most widely used. The present study was designed to develop and standardize a cost effective in-house ELISA for the detection of HbsAg and compar...

The Department of Pesticide Regulation Environmental Monitoring Branch has successfully used ELISA for analyses of pesticides in soil and water samples for over a decade using both in-house assays (Goh 1992a, 1992b, 1993, 1996) and commercially available kits (Goh 1990, 1991). We recently evaluated a commercial diazinon kit for its sensitivity, selectivity, accuracy and precision, etc. for surf...

Rapid user-friendly ABO-Rh blood typing kits (Eldon Home Kit 2511, ABO-Rh Combination Blood Typing Experiment Kit) were evaluated to determine their accuracy when used under simulated military field conditions and after long-term storage at various temperatures and humidities. Rates of positive tests between control groups, experimental groups, and industry standards were measured and analyzed ...

Three commercial latex kits, IBL, MYCO-Immune, and IMMY, for the detection of cryptococcal antigen were compared in regard to sensitivity, specificity, and height of antigen titers. A total of 218 cerebrospinal fluid and 79 serum specimens from 239 patients were included. Twenty-two patients had culture-proven disseminated cryptococcosis. Both the IBL and MYCO-Immune kits had sensitivities of 1...

Discordant results for concentrations of choriogonadotropin (HCG) in sera from five of 162 women are reported, as determined with four commercial radioassay kits: Becton-Dickinson, Clinetics, Corning, and Hybritech. Results ranged from 11.2 to 36.7 int. units/L, when assayed with the Becton-Dickinson kit, but HCG was not detectable when assayed with the other kits. None of the patients was preg...

to be avoided. Berthed and Rey (1) have also reported enormous crossreactivityof hydrocortisonehemisuccinate in the “Rianen” RIA kit for cortisol determination. An issue of Clinical Chemistry (1983;29:890-953) reports the commercial availability of(then)more than 27 cortisol RIA kits.Methodologically, these kits were divided into three groups according to the different antibodies used: antibodi...