Several reports describe the use of Qiagen columns as part of a Legionella PCR (1, 2). However, workers in two laboratories in The Netherlands have found that the Qiagen columns are contaminated with Legionella species. Nucleic acid sample preparation kits are commercially available from many different suppliers. Some of the most commonly used are the silica-based gel membrane or glass fiber fi...

We examined cytochrome oxidase (CO) activity in striate cortex of four macaque monkeys after monocular enucleation at ages 1, 1, 5, and 12 weeks. These animal experiments were performed to guide our interpretation of CO patterns in occipital lobe specimens obtained from two children who died several years after monocular enucleation during infancy for tumor. In the macaques, the ocular dominanc...

In the present work, the exergy analysis and economic study of 3 different samples of threecomponent mixtures have been investigated (ESI>1, ESI≈1, and ESI<1). The feed mixture has been tested under three different compositions (low, equal, and high contents of the intermediate component). A quantitative comparison between simple and complex configurations, considering thermally coupled, thermo...

Optimisation of peak capacity is an important strategy in gradient liquid chromatography (LC). This can be achieved by using either long columns or columns packed with small particles. Monolithic columns allow the use of long columns at relatively low back-pressure. The gain in peak capacity using long columns was evaluated by the separation of a tryptic bovine serum albumin digest with an LC-U...

Nucleic acid extraction is a basic requirement in a molecular biology laboratory. In terms of purity and yield, commercial nucleic acid extraction columns are superior; however they are expensive. We report here an efficient strategy to regenerate diverse commercial columns for several rounds without altering the binding capacity of the columns or changing the properties of the nucleic acids pu...

When an eye becomes deviated in early childhood, a person does not experience double vision, although the globes are aimed at different targets. The extra image is prevented from reaching perception in subjects with alternating exotropia by suppression of each eye's peripheral temporal retina. To test the impact of visual suppression on neuronal activity in primary (striate) visual cortex, the ...

Large-size (4-5 µm) superficially porous particles yield lower plate heights (e.g., the minimal reduced plate height or hmin ≈ 1.5) than fully porous particles of a similar size when packed into large-bore columns. This property allows for better chromatographic performance without the higher pressures required for smaller particles. This study explores the use of such particles in microfluidic...

A finite or infinite matrix A with rational entries is called partition regular if whenever the natural numbers are finitely coloured there is a monochromatic vector x with Ax = 0. Many of the classical theorems of Ramsey Theory may naturally be interpreted as assertions that particular matrices are partition regular. In the finite case, Rado proved that a matrix is partition regular if and onl...

The vacuum hot pressing (VHP) of silicon carbide monofilaments coated with nanocrystalline Ti–6Al–4V has been studied. During consolidation, very high densification rates were observed, even at temperatures and pressures well below those normally used for processing conventional Ti–6Al–4V. From the cross-sections of partially consolidated specimens, the evolution of coated fiber–fiber contacts ...

This study investigates the lateral impact responses of reinforced concrete (RC) and composite columns through dynamic nonlinear analysis using LS-DYNA. The simulation results were first validated against experimental performed earlier on four different cross sections. finite element show that LS-DYNA can predict well be used for further parametric analysis. overall resistance new is significan...