Potato virus A (PVA) particles were bombarded with thermally activated tritium atoms, and the intramolecular distribution of the label in the amino acids of the coat protein was determined to assess their in situ steric accessibility. This method revealed that the N-terminal 15 amino acids of the PVA coat protein and a region comprising amino acids 27 to 50 are the most accessible at the partic...

The Yellow Mosaic Disease is the main constraint for decreased yield in blackgram production. Coat protein gene of Mungbean yellow mosaic virus (MYMV) has been characterized and phylogenetic tree was constructed. genomic DNA from diseased plants collected it tested it’s quality quantity using a Nanodrop. isolated subjected to polymerase chain reaction begomovirus universal primers preliminary c...

Coat protein complex II (COPII) mediates formation of the membrane vesicles that export newly synthesised proteins from the endoplasmic reticulum. The inner COPII proteins bind to cargo and membrane, linking them to the outer COPII components that form a cage around the vesicle. Regulated flexibility in coat architecture is essential for transport of a variety of differently sized cargoes, but ...

Lysosomally directed receptors are concentrated at a 'bilayered' clathrin coat on the face of sorting endosomes. This coat is highly enriched in Hrs protein, which can potentially serve as an adaptor between ubiquitinated receptors and clathrin.

The region of the Pf3 virus genome encoding its major coat protein and its single-stranded DNA-binding protein is organized somewhat like the corresponding region of the fd (M13, f1) genome. Nevertheless, the major coat protein is unique among the major coat proteins of fd and the other filamentous phages studied in that it lacks a signal sequence and appears to be a direct translation product ...

The coat proteins required for budding COP-coated vesicles from Golgi membranes, coatomer and ADP-ribosylation factor (ARF) protein, are shown to be required to reconstitute the orderly process of transport between Golgi cisternae in which fusion of transport vesicles begins only after budding ends. When either coat protein is omitted, fusion is uncoupled from budding-donor and acceptor compart...

The large heterodimeric penicillin G acylase from Alcaligenes faecalis was displayed on the surface of phage fd. We fused the coding sequence (alpha subunit-internal peptide-beta subunit) to the gene of a phage coat protein. A modified g3p signal sequence was used to direct the polypeptide to the periplasm. Here we show that a heterodimeric enzyme can be expressed as a fusion protein that matur...

Šafářová D., Navrátil M. (2014): Genetic variability of the Czech Pea enation mosaic virus isolates. Czech J. Genet. Plant Breed., 50: 100–104. The Pea enation mosaic virus is an example of symbiogenesis of taxonomically unrelated PEMV-1 and PEMV-2 viruses. Partial RNA sequences, PEMV-1 coat protein, PEMV-2 movement protein gene and satellite RNA from seventeen isolates were compared with the a...

We have probed the effects of altering buffer conditions on the behaviour of two aptamer RNAs for the bacterio-phage MS2 coat protein using site-specific substitution of 2'-deoxy-2-aminopurine nucleotides at key adenosine positions. These have been compared to the wild-type operator stem-loop oligonucleotide, which is the natural target for the coat protein. The fluorescence emission spectra sh...