background coagulase-negative staphylococci (cons) are opportunistic pathogens. methicillin resistance is common in cons and may play an important role as reservoir of staphylococcal cassette chromosome mec (sccmec) for staphylococcus aureus. objectives the aim of this study was to determine molecular characteristics of nasal carriage of methicillin-resistant coagulase negative staphylococci am...

OBJECTIVE To investigate antibiotic resistance and genetic relatedness of methicillin-resistant Staphylococcus aureus (MRSA) isolated in a general hospital in Kuwait over a period from 1996 to 1998 and 2001. MATERIAL AND METHODS The isolates were characterized by antibacterial susceptibility testing, coagulase serotyping, coagulase gene polymorphism (coag-RFLP) and pulsed-field gel electropho...

Epidemiological studies have identified Staphylococcus aureus as the most common agent involved in food poisoning. However, current research highlights the importance of toxigenic coagulase-negative staphylococci (CoNS) isolated from food. The aim of this study was to characterize Staphylococcus spp. isolated from cows with bovine subclinical mastitis regarding the presence of genes responsible...

To study comprehensive toxin profiles and the chromosomal diversity of current Japanese hospital-associated meticillin-resistant Staphylococcus aureus (HA-MRSA) strains, we conducted PCR-based identification of 28 toxin genes, and staphylococcal cassette chromosome mec (SCCmec) typing and PFGE analysis of 208 MRSA strains isolated from 100 hospitals throughout Japan. Of the tested HA-MRSA strai...

BACKGROUND Staphylococcus aureus, with reduced vancomycin susceptibility, is probably under the regulation of several genes and various express phenotypes. OBJECTIVES This study aimed to investigate the phenotypic differences between vancomycin-susceptible S. aureus (VSSA), vancomycin-intermediate S. aureus (VISA), and heterogeneous VISA (hVISA) isolates. MATERIALS AND METHODS A total of 13...

Because Staphylococcus aureus strains contain multiple virulence factors, studying their pathogenic role by single-gene inactivation generated equivocal results. To circumvent this problem, we have expressed specific S. aureus genes in the less virulent organism Streptococcus gordonii and tested the recombinants for a gain of function both in vitro and in vivo. Clumping factor A (ClfA) and coag...

• A MDR Staphylococcus pseudintermedius strain CAM1 was characterized by WGS. S. belonged to st551. The also harboured tet K, which is extremely rare in this species. To determine the draft genome sequence and analyse genetic features of a clinical isolate according main typing schemes available, with special focus on antibiotic resistance. isolated from case otitis externa dog. Its identity pa...

AIMS To ascertain the clinical importance of a strain of slide coagulase positive but tube coagulase negative Staphylococcus species isolated from the blood culture of a 43 year old patient with refractory anaemia with excessive blasts in transformation who had neutropenic fever. METHODS The isolate was investigated phenotypically by standard biochemical methods using conventional biochemical...