Bovine mastitis is the most common and costly disease of dairy cattle. Cluster of differentiation 4 (CD4) is closely related to the immune response in mastitis. We quantified promoter CpG methylation levels of the CD4 gene in Chinese Holsteins with clinical mastitis (CM) and in healthy controls; these levels were quantitatively detected with bisulfite pyrosequencing assays and confirmed by clon...

Serum amyloid A (SAA) and haptoglobin (Hp) levels were determined in 25 cows suffering from amyloidosis. SAA levels in cows with amyloidosis ranged between < 0.3 and 225.8 microg/ml, with a median level of 105.1 microg/ml, and Hp levels ranged between < 20 and 1860 microg/ml, with a median level of 950 microg/ml. These levels were significantly higher than the levels observed in healthy cows (S...

Lameness in cattle is a major welfare problem and has important economic implications. It is known that lameness has a multifactorial causation; however, it is still not clear why some individuals are more susceptible than others to present foot lesions under the same environment. Social and individual behaviour is thought to play an important role. The aim of this study was to assess the possi...

BACKGROUND This study examined whether activation of innate immunity and alterations of carbohydrate and lipid metabolism precede development of subclinical mastitis (SCM). METHODS Blood samples were collected from the coccygeal vein from 100 Holstein dairy cows at -8, -4, disease diagnosis week, and +4 weeks postpartum. Six healthy cows (controls - CON) and six cows that showed clinical sign...

Milk samples were collected from quarters of 35 cows with subclinical mastitis (California mastitis test [CMT] positive and somatic cell counts [SCC] >500,000 cells/ml in individual quarter foremilk), as well as from 37 healthy controls. Compared to the levels observed in milk from healthy quarters, milk from quarters with subclinical mastitis showed elevated sodium (91.97 vs 52.93 mg/dl), chlo...

Djoković R., H. Šamanc, Z. Nikolić, S. Bošković Bogosavljević: Changes in Blood Values of Glucose, Insulin and Inorganic Phosphorus in Healthy and Ketotic Dairy Cows after Intravenous Infusion of Propionate Solution. Acta Vet. Brno 2007, 76: 533-539. The aim of the present study was to determine the degree of blood glucose utilization by peripheral tissue on the basis of changes in blood concen...

Mastitis is a frequent problem among dairy cows, reducing milk yield and increasing cull rates. Systemic therapy with the cephalosporin antimicrobial ceftiofur hydrochloride (CEF) may improve therapeutic outcomes, but the incidence of CEF violative residues has increased annually since 2011. One potential explanation is that disease status may alter the pharmacokinetics (PK) of CEF. To test thi...