Objective(s): Pseudomonas aeruginosa is one of the most important nosocomial pathogens causing a high rate of mortality among hospitalized patients. Herein, we report the prevalence of antibiotic resistance genes, class 1 integrons, major virulence genes and clonal relationship among multidrug- resistant (MDR) P. aeruginosa, isolated from four referral hospitals in the...

BACKGROUND Some genetic and phenotypic variables are associated among distinct microbial populations. OBJECTIVES The associations between multi-drug resistance (MDR) phenotypes, prevalence of antibiotic resistance integrons (ARIs), bla SHV, bla TEM and bla CTX-M gene carriage and genetic fingerprints of random amplified polymorphic DNA (RAPD), confirmed by pulsed field gel electrophoresis (PF...

The ability of bacteria to procure, sometimes rearrange, and evince acquired DNA continues to impress us-even more so if this genetic plasticity involves the sequestering of antibiotic resistance genes. The acquisition of genes in bacteria is often facilitated by transposons, integrons and archetype insertion elements. Recently however, a new element, 'orf513', has been increasingly associated ...

background and aims: acinetobacter baumannii is regarded as an important nosocomial pathogen around the world, especially in the intensive care unit that today seems to be resistant to the most antibiotics. therefore, this study aimed to trace classes 1, 2, and 3 integrin in the isolates resistant to acinetobacter baumannii. materials and methods: in this descriptive study, acinetobacter bauman...

The increase in the prevalence of carbapenem-resistant Pseudomonas aeruginosa isolates, particularly caused by the metallo-ß-lactamases (MBL) is of great concern in the clinical settings worldwide. Here, we report the detection and identification of MBL producing P. aeruginosa in our hospital (University Malaya Medical Center, Malaysia), and the characterization of MBL cassette-containing integ...

The first 13.6 kb of the mercury and multidrug resistance transposon Tn1696, which includes the class 1 integron In4, has been sequenced. In4 is 8.33 kb long and contains the 5'-conserved segment (5'-CS) and 2.24 kb of the 3'-conserved segment (3'-CS) flanking four integrated cassettes. The 3'-CS region is followed by one full copy and an adjacent partial copy of the insertion sequence IS6100 f...

The presence of class 1 integrons was investigated in 156 epidemiologically unrelated Salmonella Typhimurium isolates. Of these 156 isolates, 70 were of definitive phage type DT104 and 86 were strains of various phage type, RDNC and untypable, designated here as non-DT104 strains. Integrons were found in 47 of DT104 isolates (67.1%), while in all strains with characteristic pentaresistance (R-t...

background: acinetobacter baumannii is an important opportunistic pathogen which causes complications in hospitalized patients, especially those in icu. the aim of this study was to determine the frequency of class 1 and 2 integrons in multi-drug resistance a. baumannii and to investigate the association between the presence of integrons and antibiotic resistance patterns. methods: a total of 4...

BACKGROUND Integrons are mobile DNA elements and they have an important role in acquisition and dissemination of antimicrobial resistance genes. However, there are limited data available on integrons of Staphylococcus aureus (S. aureus) from bovine mastitis, especially from Chinese dairy cows. To address this knowledge gap, bovine mastitis-inducing S. aureus isolates were investigated for the p...

BACKGROUND Proteus mirabilis is an opportunistic pathogen, commonly associated with complicated urinary tract infections (UTIs). UTIs caused by multidrug-resistant Proteus mirabilis have increased worldwide. Multidrug-resistance of Gram-negative enteric bacteria is usually associated with class 1 integrons. PURPOSES To investigate the prevalence and characterize gene cassettes of class 1 inte...