Complexins are small soluble proteins that bind to assembling SNARE complexes during synaptic vesicle exocytosis, which in turn mediates neurotransmitter release. Complexins are required for clamping of spontaneous "mini " release and for the priming and synaptotagmin-dependent Ca(2+) triggering of evoked release. Mammalian genomes encode four complexins that are composed of an N-terminal unstr...

Peptide nucleic acid (PNA)-mediated PCR clamping (H. Orum, P. E. Nielsen, M. Egholm, R. H. Berg, O. Buchardt, and C. Stanley, Nucleic Acids Res. 21:5332-5336, 1993) was introduced as a novel procedure to selectively amplify ribosomal DNAs (rDNAs) which are not frequently found in clone libraries generated by standard PCR from complex microbial consortia. Three different PNA molecules were used;...

OBJECTIVES To report a technique of lateral pedicle control during laparoscopic radical prostatectomy that completely eliminates any form of electrocautery, ultrasonic thermal energy, clips, or bioadhesives. METHODS The technique was used in 25 men undergoing nerve-sparing laparoscopic radical prostatectomy. Our antegrade technique involves transient control of the lateral prostate pedicles w...

BACKGROUND Recently, combined intra-articular tranexamic acid (IA-TXA) injection with clamping drain method showed efficacy for blood loss and transfusion reduction in total knee replacement (TKR). However, until now, none of previous studies revealed the effect of this technique on pharmacokinetics, coagulation, and fibrinolysis. MATERIALS AND METHODS An experimental study was conducted, dur...

BACKGROUND Delayed clamping of the umbilical cord increases the infant's iron endowment at birth and haemoglobin concentration at 2 months of age. We aimed to assess whether a 2-minute delay in the clamping of the umbilical cord of normal-weight, full-term infants improved iron and haematological status up to 6 months of age. METHODS 476 mother-infant pairs were recruited at a large obstetric...

The letter by Bertolini et all is primarily addressed to three references. The first two references were two letters written to BLOOD in 1992 by myself and my colleague^.'^^ These letters written in 1992 were not written to draw attention to the loss of hemoglobin, but to draw attention to ethical, medical, and legal issues revolving around the deliberate taking of blood from a n e w b ~ r n . ...

Cerebral Blood Flow Measurement as a Safeguard During Carotid Endarterectomy • Intraoperative measurement of regional cerebral blood flow (rCBF) by the Xe intracarotid injection technique was performed in 17 patients operated on for stenosis of the internal carotid artery. Mean rCBF decreased from 51 ml/100 gm x minute before endarterectomy to 30 ml/100 gm x minute when the internal carotid art...