Detection of cardiac troponin I (cTnI) in patients suspected of having an acute coronary syndrome is highly predictive for an adverse outcome. We evaluated a bedside test for cTnI that uses a polyclonal capture antibody and two monoclonal indicator antibodies. Clinical studies were performed in patients with acute coronary syndrome and patients with chest pain but no evidence of acute myocardia...

The enzyme creatine kinase (CK) [EC 2.7.3.2] is a dimeric molecule consisting of two separate subunits Band M, giving rise to three isoenzymes CK-BB, CK-MB and CK-MM. Most of the CK found in the plasma of normal individuals is the MM isoenzyme derived from skeletal muscle, while the CK-MB fraction originates predominately from cardiac muscle. Normally there is little, if any, CK-BB isoenzyme pr...

Sir, Creatine Kinase (CK) E.C. 2.7.3.2. is separable into three different isoenzymes by a number of methods. Each isoenzyme is a dimer composed of two subunits (Bor M) each having a relative molecular mass of 40,000. These isoenzymes are widely distributed in tissues, CK-MM and CK-MB are found mainly in the skeletal and heart muscles and CK-BB in G.l. tract and the brain with small amounts also...

The effect of i.v. nitroglycerin administration on indexes of infarct size was examined in 31 patients with acute myocardial infarction. Serial creatine kinase (CK) and CK-MB isoenzyme determinations were used to calculate infarct size. Twenty-nine patients served as controls. Two subgroups of the study group were formed to evaluate differences between early and late intervention. In the first ...

In their state-of-the-art paper on biomarkers in acute cardiac disease (1), Dr. Jaffe and colleagues list creatine kinase-MB (CK-MB) as a “potentially outdated marker.” However, CK-MB has a specific utility in the diagnosis of reinfarction (2), and it cannot be replaced by the cardiac troponins for this purpose. By following up the time course of rise and fall of CK-MB, an interruption in the p...

BACKGROUND Creatine kinase MB (CK-MB), and cardiac troponin I (cTnI) are important biomarkers for the diagnosis and rule-out of acute myocardial infarction (AMI) of patients who presented to the emergency department (ED) with chest pain. With new rapid ED assessment protocols, there is increasing pressure to produce results with a short turnaround time (TAT), and point-of-care (POC) testing is ...

Limitations of creatine kinase-MB (CK-MB) have led to alternative biochemical markers, including troponin T (TnT), to detect myocardial necrosis. Limited data are available regarding the predictive value of this new marker in patients with chest pain of uncertain etiology. Therefore, we prospectively compared CK-MB and TnT in a broad population with suspected acute coronary syndromes, including...

During the last three years we and other have observed discrepancies between results for creatine kinase isoenzyme MB as measured with the mechanized ion-exchange chromatographic method in the Du Pont aca and those by other techniques. These observations prompted us to investigate the influence of the matrix on the Du Pont CK-MB assay. We conclude that, apart from possible interferences by CK-M...

The development of rapid, automated, and accurate laboratory testing for creatine kinase MB (CK-MB) revolutionized the treatment of patients with acute cardiac events in the 1970s and 1980s.1 To clinicians, CK-MB values augmented a thorough history, physical, and ECG findings, and elevations rapidly became the gold standard for identifying cardiac injury.1 CK-MB allowed earlier diagnosis of acu...