نتایج جستجو برای: chromomycine a 3 staining

تعداد نتایج: 13826109  

Journal: :avicenna journal of medical biotechnology 0

conjugation of monoclonal antibodies to super paramagnetic nanoparticles is an effective method for cancer diagnosis and treatment. in this study the humanized anti her2/neu monoclonal antibody- herceptin- was conjugated to super paramagnetic iron oxide (spio) nanoparticles using edc method. the concentration of the conjugated antibodies was measured by bradford assay. the antibody-nanoparticle...

Background: Hyperuricemia contributes to kidney injury, characterized by tubular injury with epithelial–mesenchymal transition (EMT). Wnt5a/Ror2 signaling drives EMT in many kidney pathologies. This study sought to evaluate the involvement of Wnt5a/Ror2 in hyperuricemia-induced EMT in kidney tubular injury.Methods: A hyperuricemia model was performed in male Swiss background mice (3 months old,...

Journal: :Frontiers in Oncology 2021

Mitochondria play important roles in regulating cell bioenergetics status and reactive oxygen species (ROS) generation. ROS-induced mitochondrial damage is among the main intracellular signal inducers of autophagy. Autophagy a cellular catabolic process that regulates protein organelle turnover, while selective form autophagy, mitophagy, specifically targets dysfunctional degradation. This stud...

Journal: :Investigative ophthalmology & visual science 1981
L Carter-Dawson T Kuwabara J G Bieri

The effects of moderate-intensity light (150 to 200 ft-cd) on retinal structure were compared between retinol-adequate and retinol-deficient rats after 1 to 6 days of light exposure during the 12 hr light phase of the cycle. Both damage to the outer segments and loss of photoreceptor cells were accelerated in retinol-adequate rats. Outer segments in retinas of retinol-adequate rats showed an ab...

Journal: :The Journal of Cell Biology 1989
S Jaken K Leach T Klauck

We have used immunocytofluorescence techniques to determine the subcellular distribution of the Ca2+, phospholipid-dependent protein kinase, protein kinase C (PKC). Using monoclonal antibodies that are specific for Type 3 (alpha) PKC, we have determined that there are least two pools of PKC in normal rat embryo fibroblasts (REF52 cells): diffuse cytoplasmic and fiber-associated. Extraction with...

2006
D. J. GOLDSTEIN

Methods are proposed for the estimation of the rate, activation energy, heat, and affinity of staining of histological sections, and approximate results are given for the staining of mucin, mast-cell granules, chromatin, cytoplasmic ribonucleic acid (RNA), cartilage matrix, and other structures by azure A. The half-staining time tj is the time taken by a substrate under given staining condition...

Journal: :Jurnal Teknologi Laboratorium 2022

One of the complications Diabetes Mellitus (DM) is damage to kidneys. Using L. plantarum, it can reduce glucose levels so that also possible repair damaged kidney structures. The process repairing structure by L plantarum be seen microscopically in glomerulus with Alcian Blue staining (AB). purpose study was determine differences microscopic results DM rat kidneys after treatment AB staining. s...

Journal: :The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 1997
T Suzuki K Fujikura T Higashiyama K Takata

We examined five nucleic acid binding fluorescent dyes, propidium iodide, SYBR Green I, YO-PRO-1, TOTO-3, and TO-PRO-3, for nuclear DNA staining, visualized by fluorescence and laser confocal microscopy. The optimal concentration, co-staining of RNA, and bleaching speeds were examined. SYBR Green I and TO-PRO-3 almost preferentially stained the nuclear DNA, and the other dyes co-stained the cyt...

Journal: :Journal of clinical pathology 2004
A N Kalof D Tam B Beatty K Cooper

BACKGROUND Recent studies have reported CD10 expression in myoepithelial cells (MEC) of the breast, supporting its use as a marker to help distinguish invasive breast carcinoma (IC) from ductal carcinoma in situ (DCIS). AIM To compare the effectiveness of CD10 with smooth muscle myosin heavy chain (SMMHC) in the detection of MEC in benign and malignant breast lesions. METHODS Histological m...

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