Tissue factor (TF) pathway inhibitor I (TFPI) is the physiological inhibitor of TF-induced blood coagulation. Circulating blood contains full-length TFPI and TFPI truncated at the C-terminal end. Previous studies have shown that full-length TFPI exerts a stronger anticoagulant effect on diluted prothrombin time (DPT) than truncated TFPI, and it has been suggested that full-length TFPI is biolog...

Abstract Background The irrational use of carbapenems in the last years lead to emergence carbapenem-resistant Enterobacteriaceae (CRE). This study aimed at determining prevalence CRE intestinal carriage among admitted patients a tertiary care hospital Egypt, characterize carbapenemase-producing genes and identify possible risk factors colonization. One hundred rectal swabs were collected from ...

Golden Gate Assembly is a powerful synthetic biology tool, which utilizes Type IIS enzymes for unidirectional assembly of multiple DNA fragments. The simplicity of its DNA assembly and the exchangeability of standard parts greatly facilitate the generation of combinatorial assembly libraries. Currently there are two popular Golden Gate Assembly frameworks that allow multigene augmentation (MoCl...

The traditional measurement methods of 3 kinds of aluminum species and the total aluminum content in polyaluminum solutions are Al-Ferron timed complex spectrophotometry and EDTA chelatometry respectively. But eachof these methods has different deficiencies. In this paper, microwave digesting technical had be coupled with the radiate Ferron spectrophotometry to rapid mensurate the concentration...

In a preliminary study, known staphylococcus (n = 86) and other microbial (n = 12) isolates were plated on three chromogenic media, SaSelect (Bio-Rad, Hercules, CA, USA), CHROMagar Staph. aureus (CHROMagar Microbiology, Paris, France), and S. aureus ID (bioMérieux, Marcy l'Etoile, France). The sensitivities of all the media to detect Staphylococcus aureus after 24 h of incubation were high (100...

Recently, systems have been developed to create total laboratory automation for clinical microbiology. These systems allow for the automation of specimen processing, specimen incubation, and imaging of bacterial growth. In this study, we used the WASPLab to validate software that discriminates and segregates positive and negative chromogenic methicillin-resistant Staphylococcus aureus (MRSA) pl...