The application of genetically encoded biosensors enables the detection small molecules in living cells and has facilitated characterization enzymes, their directed evolution engineering (natural) metabolic pathways. In this work, LuxAB biosensor system from Photorhabdus luminescens was implemented Escherichia coli to monitor enzymatic production aldehydes primary alcohols carboxylic acid subst...

Trends in biosensor research and development are described. Micromachining techniques have been applied to construct biosensor systems. Several detection units for flow injection analysis have been fabricated. An electrochemical flow cell was fabricated, and both an enzyme immobilized column and an electrochemical detector were integrated. A chemiluminescence detector was also fabricated. Biose...

Since the report of Brahn (1) that very low catalase values are found in the livers of human beings dead of various forms of cancer, many papers have appeared on the effect of a growing tumor upon enzyme systems in liver tissue. Greenstein (4) has shown that in rats bearing transplanted hepatoma 31 or Jensen sarcoma, or in mice bearing various transplanted tumors, the activity of the liver cata...

background: many in vitro studies suggested that choline incorporation into many types of tumors is related to cell proliferation. whether in vivo choline incorporation is related also to cell proliferation or not was my question. therefore, the aim of this pilot study was to investigate the relationship of in vivo [methyl- 14c]-choline incorporation and in vivo [methyl-3h]- thymidine incorpora...

In the search for useful and renewable chemical building blocks, 5-hydroxymethylfurfural (HMF) has emerged as a very promising candidate, as it can be prepared from sugars. HMF can be oxidized to 2,5-furandicarboxylic acid (FDCA), which is used as a substitute for petroleum-based terephthalate in polymer production. On the basis of a recently identified bacterial degradation pathway for HMF, ca...

Weanling male Sprague-Dawley rats were fed either a nutritionally complete synthetic diet (Diet 1) or a diet marginally deficient in choline and methionine, and lacking folacin (lipotrope deficient, Diet 2) to determine the role of hepatic mixed-function oxidase metabolism of aflatoxin B1 (AFB1) in the Diet 2-induced enhancement of AFB1 hepatocarcinogenesis previously reported. Hepatic microsom...

The superoxide (O2-)-forming NADPH oxidase of resting macrophages can be activated in a cell-free system by certain anionic amphiphiles, such as sodium dodecyl sulfate (SDS). O2- production requires the cooperation of membrane-associated and cytosolic components. The membrane component can be solubilized by octyl glucoside yielding a highly active oxidase preparation. High performance gel filtr...