Considerable evidence exists which is consistent with the picture that the chloroplast system of E1uglcna is unider autonomous genietic control. This includes studies of ultraviolet inactivationi anid photoreactivation of chloroplast inheritanice (7, 10. 13, 14) and the demonlstration of a unique species of chloroplast-associated DNA (3, 4, s5, 9, 11). Evidence has been presented for distinct c...

Chloroplast research takes significant advantage of genomics and genome sequencing, and a new picture is emerging of how the chloroplast functions and communicates with other cellular compartments. In terms of evolution, it is now known that only a fraction of the many proteins of cyanobacterial origin were rerouted to higher plant plastids. Reverse genetics and novel mutant screens are providi...

Chloroplast DNA of the green alga Chlamydomonas reinhardtii is maternally inherited. Methylation mapping directly revealed that, before mating, chloroplast DNA of maternal (mating type plus; mt(+)) gametes is heavily methylated whereas that of paternal (mating type minus; mt(-)) gametes is not. Indirect immunofluorescence analyses with anti-5-methylcytosine mAbs visually showed methylation to o...

In higher plants, embryo development originated from fertilized egg cell is the first step of the life cycle. The chloroplast participates in many essential metabolic pathways, and its function is highly associated with embryo development. However, the mechanisms and relevant genetic components by which the chloroplast functions in embryogenesis are largely uncharacterized. In this paper, we de...

The chloroplast is essential for photosynthesis and the production of hormones and metabolites. As a consequence, its biogenesis and development needs to be coordinated with seedling growth to ensure optimal rates of photosynthesis without oxidative damage upon seedling emergence. Studies into the processes of biogenesis and development of chloroplasts have shown how important chloroplast devel...

Dinoflagellates are an economically and ecologically important eukaryotic algal group. The organization of their chloroplast genome appears to be radically different from that in plants and other algae. The gene content has been dramatically reduced in dinoflagellates, with the large-scale transfer of genes to the nucleus. Most of the remaining genes encode subunits of Photosystems I and II, th...

The effects of 24-h exposure to spectinomycin (100 microgram/ml) and ethidium bromide (1 microgram/ml) on the accumulation of chloroplast and mitochondrial rRNAs and on organelle ultrastructure were studied in greening cells of Ochromonas danica. Cells treated with ethidium bromide for 24 h divide at the same rate as controls but contain less than one third the normal amount of mitochondrial rR...

The rate of appearance of labelled RNA in the chloroplast and mitochondria as compared with the rate in the remaining cytoplasm was studied in the unicellular flagellate, Ochromonas danica, by electron-microscope autoradiography. Greening cells were labelled withuridine5,6['H] for a short (30 min) and a long (2 h) interval and the concentration of label, expressed as grains/unit area, determine...

It has been reported that exogenously supplied thymidine and thymidine analogues, e.g. 5-bromodeoxyuridine, are specifically incorporated into chloroplast (B-component) D N A in Chlamydomonas reinhardtii (10). The chloroplast-specific incorporation is found in vegetative cells, but this specificity is lost during meiosis when both nuclear D N A and chloroplast D N A can be labeled by radioactiv...

Light plays a key role in the regulation of many physiological processes required for plant and chloroplast development. Plant cryptochromes (crys) play an important role in monitoring, capturing, and transmitting the light stimuli. In this study, we analyzed the effects of CRY2 overexpression on transcription of tomato chloroplast genome by a tiling array, containing about 90 000 overlapping p...