Chitin Nanocrystals nanomaterials are extracted from crab shells to stabilize bicontinuous emulsions by intra-phase jamming at ultra-low concentrations. In article number 2300686, Long Bai, Orlando J. Rojas, and co-workers show the merits of bio-based nanoparticles in forming complex multiphase systems, offering a new pathway achieve advanced hierarchical multiscale materials.

Six kinds of chitinous materials have been used as sole carbon/nitrogen (C/N) sources for producing α-glucosidase inhibitors (aGI) by Paenibacillus sp. TKU042. The aGI productivity was found to be highest in the culture supernatants using demineralized crab shell powder (deCSP) and demineralized shrimp shell powder (deSSP) as the C/N source. The half maximal inhibitory concentration (IC50) and ...

Mouse acidic mammalian chitinase (AMCase) plays important physiological roles in defense and nutrition. AMCase is composed of an N-terminal catalytic domain (CatD) and a C-terminal chitin-binding domain (CBD). We expressed CatD of mouse AMCase as a recombinant fusion protein with Protein A and V5-His in Escherichia coli (Protein A-CatD-V5-His), evaluated its functional properties and compared t...

The chitinolytic microorganisms, isolates 18 and 19, were selected from soil samples around a region of frozen shrimp factory in Chonburi province. These isolates had high potential for using chitin as a carbon source from the chitin broth, which was composed of 1.0% chitin, 1.0% NH4NO3 and 3.0% yeast extract. The cultures were grown at 200 rpm, 30∞C and initial pH of 6.8-7.0 for 7 days. It was...

Chitinase production by newly isolated Serratia marcescens B4A was optimized following Taguchi’s array methods. Twenty-three bacterial isolates were screened from shrimp culture ponds in the South of Iran. A chitinase-producing bacterium was isolated based on it’s ability to utilize chitin as the sole carbon source. The isolate designated as B4A, was identified as Serratia marcescens based on i...

Shellfish is one of Indonesia's fishery commodities which increasing every year, blood clams and feather shells, are easily found in Southeast Asia East Asia. also have great potential so that it has an impact on the waste allowed to accumulate without any handling pollution exists environment society. One alternative use from shells a high value isolate into chitosan. The purpose this study wa...

Twelve bacterial strains isolated from shrimp farming ponds were screened for their growth activity on chitin as the sole carbon source. The highly chitinolytic bacterial strain was detected by qualitative cup plate assay and tentatively identified to be Cohnella sp. A01 based on 16S rDNA sequencing and by matching the key morphological, physiological, and biochemical characteristics. The culti...

The Clostridium paraputrificum chiB gene, encoding chitinase B (ChiB), consists of an open reading frame of 2,493 nucleotides and encodes 831 amino acids with a deduced molecular weight of 90,020. The deduced ChiB is a modular enzyme composed of a family 18 catalytic domain responsible for chitinase activity, two reiterated domains of unknown function, and a chitin-binding domain (CBD). The rei...

In this study, an easy synthesized method for preparation of chitosan/iron oxide nanocomposite as a bio-sorbent has been applied. Analytical techniques such as Fourier transform infrared spectroscopy, X-ray diffraction; Field emission scanning electron microscopy and transmission electron microscopy were utilized to survey of morphological structure and the functional groups characterization. T...