Part of the porcine rotavirus outer capsid protein VP7 containing all the three antigenic regions was expressed as a chimeric protein with bacterial alkaline phosphatase (AP) in E. coli. The construct contains an ompF promoter, the DNA encoding the signal sequence and the first 12 amino acids of mature OmpF, part of vp7 and the DNA encoding mature AP. The chimeric protein is stable, retains the...

clostridium botulinum type a (bont/a) produces a neurotoxin recently found to be useful as an injectable drug for the treatment of abnormal muscle contractions. the catalytic domain of this toxin which is responsible for the main toxin activity is a zinc metalloprotease that inhibits the release of neurotransmitter mediators in neuromuscular junctions. a cell penetrating cationic peptide, tat, ...

Radioimmunotherapy (RIT) of Non-Hodgkin’s lymphoma (NHL) is said to be more advantageous compared to unlabelled therapeutic antibodies. To this date, radiolabelled murine anti-CD20 mAbs, Zevalin® and Bexxar® have been approved for imaging and therapy. A preparation containing rituximab, chimeric mAb radioimmunoconjugate suitable for Lu-177 labeling, could provide better imaging and therapeutic ...

A panel of chimeric carrier proteins was developed and screened for functional activity with essential enzymes involved in carrier protein-mediated biosynthesis. Regions on either side of the recognition helix II within three carrier proteins (CPs) from distinct biosynthetic pathways were swapped in all combinations to generate 24 mutated CPs. This panel of chimeric carrier proteins was tested ...

Based on the protein sequence deduced from a cDNA clone, it has been proposed that the maize bt1 locus encodes an amyloplast membrane metabolite translocator protein (Sullivan, T. D., Strelow, L. I., Illingworth, C. A., Phillips, R. L., and Nelson, O. E., Jr. (1991) Plant Cell 3, 1337-1348). The present work provides further evidence for this hypothesis by showing that the gene product of Bt1 c...

Ferritins are ideal nanoparticles as drug delivery systems due to their hollow-sphere structure and the ability target specific receptors on cell surface. Here, we develop characterize a new ferritin derived from chimeric humanized A. fulgidus one, already designed recognize TfR1 receptor. Starting synthetic gene of this protein, replaced two positively charged amino acids with alanine residues...

The number of biologic drugs available for the treatment psoriasis continue to expand. However, being biological proteins and thus potentially immunogenic, there is evidence that anti-drug-antibodies develop against various therapeutic currently utilised. Although chimeric antibodies contain elements parental monoclonal are anti-drug occur even if a fully human protein these can impact on clini...