background and aim: in most infections and diseases, to cure and decrease the effect of disease, induction and increasing power of immuno system are necessary. nigella sativa is a plant that has been used for treatment of many diseases. in this survey, the effect of nigella sativa is surveyed in strengthening of monocyte phagocytosis so that the use and mechanism of this plant for strengthening...

Chronic and recurrent infections occur commonly in both type 1 2 diabetes (T1D, T2D) increase patient morbidity mortality. Neutrophils are professional phagocytes of the innate immune system that critical pathogen handling. Neutrophil responses to infection dysregulated diabetes, predominantly mediated by persistent hyperglycaemia; chief biochemical abnormality T1D T2D. Therapeutically enhancin...

Objective(s) Motility plays a major role in pathogenicity of Helicobacter pylori, yet there is  scarce data regarding its chemotactic behaviour. The present study was designed to investigate the chemotactic responses of local isolates of H. pylori towards various sugars, amino acids, as well as some other chemical substances. Materials and Methods Chemotaxis was assayed by a modified Adler’s...

It is presently accepted that emotional disturbances lead to immune system impairment, and that therefore their treatment could restore the immune response. Thus, the aim of the present work was to study the effect of an acupuncture treatment, designed specifically to relieve the emotional symptoms stemming from anxiety, on several functions (adherence, chemotaxis, phagocytosis, basal and stimu...

The purpose of this study was to use a chemically defined medium to identify essential substances and optimal conditions for the liquid storage of neutrophils at 22 degrees C. Several commercially available synthetic media were evaluated: L-15, McCoy's 5a, M199, minimum essential medium, Dulbecco's MEM, NCTC135, and RPMI 1640. Proteins, glucose, pH, and neutrophil concentration were systematica...

Free radicals and proinflammatory cytokines from phagocytes have been implicated in the pathogenesis of endotoxic shock, a disease with high mortality caused by Gram-negative bacterial endotoxin. In the present study, male BALB/c and Swiss mice received intraperitoneally lipopolysaccharide (LPS) at 100 mg/kg and 150 mg/kg, respectively, that led to a lethal endotoxic shock (100 % of mortality b...

Using a new in vitro method of measuring the chemotaxis of polymorphonuclear leukocytes from peripheral blood, a chemotactic index has been calculated. The mean chemotactic index of 320 in 24 patients with definite rheumatoid arthritis, was significantly less (P < 0.0005) than the mean of 555 in 24 normal controls matched for age and sex. The mean chemotactic index of 435 in eight patients with...

Gaucher disease patients are occasionally affected by chronic or fulminant infections. Since Gaucher cells originate from tissue phagocytes, we studied the functional implications of glucocerbroside accumulation on phagocytes in Gaucher disease patients. Circulating monocytes and granulocytes from nine type I Gaucher disease patients, and matched controls, were studied. Evaluation of phagocytic...

The social amoeba Dictyostelium discoideum is a commonly used eukaryotic model organism for the study of cell division, chemotaxis, differentiation, phagocytosis, and other cellular processes. Electroporation an effective efficient method delivering plasmid DNA into D. discoideum, invaluable tool studying intracellular technology readily available but often prohibitively expensive. Although sev...