The chemiluminescence of fluorescein derivatives is greatly enhanced when they are conjugated to proteins. We exploited this property in devising a chemiluminescence immunoassay for rubella antibodies. In this sequential "sandwich"-type assay, tubes coated with rubella antigen are used for antibody separation and goat antibody to human IgG conjugated with fluorescein isothiocyanate is used for ...

It has been suggested that oxygen-derived free radicals may contribute to the myocardial injury associated with ischemia and reperfusion. As the presence of enhanced free radical generation is a prerequisite for such damage, several techniques have been used to provide evidence of increased oxygen free radical production during reperfusion; however, all such techniques have substantial limitati...

AIMS To study the chemiluminescence response in polymorphonuclear leucocytes (PMNL) at different stages of maturity and the opsonic capacity of sera with defined titres of anti-capsular type III antibodies, after exposure to serotype III group B streptococci (GBS). The influence of GBS type III capsule expression on PMNL chemiluminescence response was also investigated. METHODS Two clinical i...

The chemiluminescence (CL) arising from the reaction of bis(2,4,6-trichlorophenyl)oxalate (TCPO) with H2O2 in the presence of a novel blue fluorescer, furandicarboxylate derivative, has been studied. The relationship between the CL intensity and concentrations of peroxyoxalate, sodium salicylate, H2O2 and furandicarboxylate derivative as fluorescer has been investigated. Kinetic parameters for ...

Objective: To analyze the dynamic evaluation of chemiluminescence, colloidal gold, and immunofluorescence chromatography in detecting antibodies COVID-19 patients within four weeks infection, to provide evidence for clinical application. Method: 74 with confirmed SARS-COV-2 infection local area were selected as experimental group, while 231 negative results but not vaccinated Covid19 vaccine co...

Compared with Au@SNAzyme, SiO 2 –SNAzyme@ABEI higher catalytic activity, which can be applied chemiluminescence detection of miDNA-499.

the chemiluminescence (cl) arising from the reaction of bis(2,4,6-trichlorophenyl)oxalate (tcpo) with h2o2 in the presence of a novel blue fluorescer, furandicarboxylate derivative, has been studied. the relationship between the cl intensity and concentrations of peroxyoxalate, sodium salicylate, h2o2 and furandicarboxylate derivative as fluorescer has been investigated. kinetic parameters for ...