DNA detection technologies have an increasing importance in our everyday lives, with applications ranging from microbial diagnostics to forensic analysis, food safety evaluation, and environmental monitoring. Currently, as the associated costs decrease, DNA diagnostic techniques are routinely used in research laboratories, in clinical and forensic practice. The first aim of this thesis is to un...

OBJECTIVE To inspect cigarettes with a triple granular filter for charcoal granules on the cut filter surface and, if present, to determine whether the charcoal granules on the filter are released during smoking. DESIGN 400 Lark cigarettes in 20 packs were examined individually by each of three investigators for the presence of charcoal granules on the cut surface of the cellulose acetate fil...

Although many endo-ß-1,4-glucanases have been isolated in invertebrates, their cellulolytic systems are not fully understood. In particular, gastropod feeding on seaweed is considered an excellent model system for production of bioethanol and renewable bioenergy from third-generation feedstocks (microalgae and seaweeds). In this study, enzymes involved in the conversion of cellulose and other p...

We previously reported the development of a cellulose fiber based blood filter device containing immobilized protamine (termed protamine filter) that could be used to control both heparin- and protamine-induced complications during extracorporeal therapy. To achieve enhanced heparin adsorption on the fibers, we examined the possibility of utilizing the poly-L-lysine based amplification method t...

Background Presently, enzymes still constitute a major part of the cost of biofuel production from lignocellulosic biomass. Processive endoglucanases, which possess both endoglucanase and exoglucanase activity, have the potential to reduce the costs of biomass saccharification when used together with commercial cellulases. Therefore, the exploration of new processive endoglucanases has attracte...

An eco-friendly time indicator (TI) was developed using cellulose-based materials. The TI comprises a dye, copy paper, nanofibrillated cellulose (NFC) film, and filter papers coated with NFC slurry and/or paraffin-free biowax. A suitable dye its conditions were determined by observing the solutions migration pattern at different concentrations. Commercial prepared, rate, depending on physical p...

Corncob is one of the industrial waste has cellulose content of 39.1 wt%, which makes it has high potential to be a raw material in the production of cellulose nanocrystal. Corncob was delignificated with 3.5 wt% HNO3 and NaNO2 10 mg, precipitated process with 17.5 wt% NaOH, and bleached with 10 wt% H2O2. Cellulose nanocrystal was obtained by hydrolysis using 45 wt% H2SO4. Corncob and cellulose...

Abstract Improved understanding of cellulose swelling mechanism is beneficial for increasing the hydrolysis efficiency cellulosic substrates. Here, we report a family 5 glycoside hydrolase ArCel5 isolated from cellulose-gelatinizing fungus Arthrobotrys sp. CX1. exhibited low specific activity and high capability, which suggested that this protein might function as an accessory protein. Homology...

We present a novel strategy based on the immobilization of palladium nanoparticles (Pd NPs) on filter paper for development of a catalytic system with high efficiency and recyclability. Oleylamine-capped Pd nanoparticles, dispersed in an organic solvent, strongly adsorb on cellulose filter paper, which shows a great ability to wick fluids due to its microfiber structure. Strong van der Waals fo...