Several proteins were extracted from the purified cell walls of suspension-cultured sugar beet cells with 0.5% EDTA (pH 6.8) after prior extraction of the walls with 0.5% deoxycholate and then with 2 molar NaCl. Two abundant proteins (P-I and P-II protein) were separately purified to homogeneity by procedures that included fractionation with ammonium sulfate, column chromatography on DEAE-cellu...

The routine application of human pluripotent stem cells (hPSCs) and their derivatives in regenerative medicine and drug discovery will require the constant supply of high cell numbers generated by robust and well-defined processes. Previous studies showed that mTeSRTM1 is the most supportive medium for a novel hPSC culture strategy, namely hPSC propagation and expansion of 3-dimensional (3D) ag...

Bovine lactoferrin (bLf) is an iron-binding secretory protein present in breast milk, mucosal secretions, and the secondary granules of neutrophils. Although bLf has multiple functions, including antimicrobial and immunomodulatory activities, its effect on neuronal cells is not fully understood. We report that bLf prevents cell adhesion of PC12 cells and allows them to be cultivated in suspensi...

The low-affinity nerve growth factor receptor p75 is a stratified epithelial stem/progenitor marker of human epithelia. We found OM-1, squamous cell carcinoma (SCC) line, showed distinct cells with cluster, especially located at the center growing colony in monolayer culture. A cluster was surrounded by cytokeratin 14- and 13-expressing that settled outer margin colony. OM-1 were also capable f...

The cell suspension cultures were established from leaf, fruit and root explants of Indian Mulberry (Morinda citrifolia) for the production of medicinally important secondary metabolites, anthraquinones, flavonoids and phenolics and the effect of subculture on these secondary metabolites was studied. The MS medium formulated with NAA, BA and Kinetin was found to be effective to produce anthraqu...

Pectic oligomers induced a rapid, transient increase in ethylene biosynthesis when added to pear cells in suspension culture. The rate of ethylene biosynthesis increased within 30 to 40 minutes after oligomer addition, reached a maximum between 90 and 120 minutes after addition, and then decreased to basal rates of synthesis. Both the rapid increase and decrease in biosynthesis appear to be pre...

Plant cell aggregates have long been implicated in affecting cellular metabolism in suspension culture, yet the rigorous characterization of aggregate size as a process variable and its effect on bioprocess performance has not been demonstrated. Aggregate fractionation and analysis of biomass-associated product is commonly used to assess the effect of aggregation, but we establish that this met...

background: while articular chondrocytes are among those appropriate candidates for cartilage regeneration, the cell dedifferentiation during monolayer culture has limited their application. several investigations have indicated the usefulness of alginate, but the topic of proliferation and differentiation of chondrocytes in alginate culture has still remained controversial. methods: rat articu...

When murine L1210 leukemia cells growing in suspension culture are deprived of tryptophan, there is a rapid fall in DNA and histone synthesis and a less rapid fall in whole cellular protein and non-histone nuclear protein synthesis. Initially, the S phase of the cell cycle becomes prolonged, but subsequently the cells become defective in their ability to reinitiate S phase. By 24 hr complete gr...

Recombinant production of complex eukaryotic proteins for structural analyses typically requires a profound screening process to identify suitable constructs for the expression of ample amounts of properly folded protein. Furthermore, the evaluation of an optimal expression host has a major impact on protein yield and quality as well as on actual cost of the production process. Here we present ...