نتایج جستجو برای: cell patch clamp

تعداد نتایج: 1724747  

2012
Zhi-Hong Wang Hidetaka Uno Noriko Takada Obuliraju-Senthil Kumar Toru Ishizuka Hiromu Yawo Tsuneo Urisu

Recently we have developed 4 channel incubation type planar patch clamp biosensor with polymethylmethacrylate (PMMA) substrate by using both-side hot embossing, focused ion beam micro-fabrication and surface modification by extra cellular matrix. We have evaluated the performance by using the light-gated ion-channel protein, channelrhodopsin wide receiver (ChRWR) expressed on HEK293 cell. Obser...

Journal: :Circulation research 1994
M Kaibara S Mitarai K Yano M Kameyama

The present study investigated the possible involvement of a Na(+)-H+ antiporter in the regulation of L-type Ca2+ channels by angiotensin II (Ang II) in isolated rabbit ventricular cardiac myocytes by using both cell-attached and whole-cell patch-clamp current recording techniques. In cell-attached patch-clamp current recordings, an increase in the open-state probability of the Ca2+ channel (14...

2015
Alex Rafacho Manuel Castellano-Muñoz Paloma Alonso-Magdalena Esperanza Irles Melisa Bello Jean Vetorazzi Beatriz Merino Sergi Soriano Pau Iborra Antonia Ruiz Raúl M Luque Angel Nadal Ivan Quesada

Results Using insulin and glucagon secretion protocols with fresh islets isolated from C57BL6 mice, we observed that CORT reduced the glucose-stimulated insulin secretion (GSIS) in a similar magnitude from that of SST (p<0.01), an effect mediated by SST-R5 receptor. Glucagon secretion in response to 0.5 mM glucose was completely abrogated in the presence of CORT (p<0.001), as well as for SST. B...

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