نتایج جستجو برای: cdna synthesis and real
تعداد نتایج: 16967463 فیلتر نتایج به سال:
Background: Recent researches have demonstrated that human T-cell immunoglobulin mucin 1 (TIM-1) glycoprotein plays important roles in regulation of autoimmune and allergic diseases, as well as in tumor immunity and response to viral infections. Therefore,targeting TIM-1 could be a potential therapeutic approach against such diseases. Objectives: In this study, we aimed to express TIM-1 protein...
to teach english adequately, qualified teachers are needed. unfortunately, there are still teachers who are teaching english without an academic degree in tefl. it is necessary for teachers to have a major in english to have subject knowledge, but it is not sufficient. teachers need to have an understanding of the social, cultural, moral, ethical, and pedagogical issues of education and practic...
abstract the present thesis seeks to critically read george orwell’s nineteen eighty-four and animal farm in the light of louis althusser’s thought and theory. the present thesis proceeds to examine and demonstrate althusser’s major concepts including ideological state apparatuses (isas), repressive state apparatuses (rsas), the structure, the subject, and ideological interpellation which r...
there are many approaches for solving variety combinatorial optimization problems (np-compelete) that devided to exact solutions and approximate solutions. exact methods can only be used for very small size instances due to their expontional search space. for real-world problems, we have to employ approximate methods such as evolutionary algorithms (eas) that find a near-optimal solution in a r...
foot-and-mouth disease (fmd) is a severely contagious viral disease that mainly affects cloven-hoofedlivestock and wildlife. this study quantifies the cytokines mrna expression of vaccinated guinea pigs withfmd type o inactivated vaccine. blood samples were collected from eight guinea pigs at 7 and 28 days after thefirst vaccination. extracted mrnas were reverse-transcribed into cdna and analyz...
abstract this study aimed at investigating the effect of bilingual teaching of cognate words (persian-english) on iranian upper intermediate efl learners’ knowledge of lexical development. for this purpose,100 subjects participated in this study out of which 40 learners were selected for this study and they were assigned into two groups, control and experimental. cross-language cognates (wor...
The isolation of high quality RNA is a crucial technique in plant molecular biology. The quality of RNA determines the reliability of downstream process like real time PCR. In this paper, we reported a high quality RNA extraction protocol for a variety of plant species. Our protocol is time effective than traditional RNA extraction methods. The method takes only an hour to complete the procedur...
وارونوشتاز مقاوم به دما (trt ) اهمیت زیادی جهت تولید cdna دارد. ژن trt با توانایی تولید این آنزیم، از باکتری geobacillus stearothermophilus strain10 استخراج و توسط حامل بیانی pet28-a در میزبان escherichie coli کلون شد و شرایط رشد این میزبان هترولوگ مورد بهینه سازی قرار گرفت. زیست توده ها جمع آوری شد.عصاره سلولی تهیه، آنزیم مورد نظر تخلیص و غلظت پروتئینی آن سنجیده شد. وارونوشتاز حاصل، جهت rt-pcr...
Ribosomal protein (rp) S6 is the substrate of ribosomal protein S6K (S6 kinase) and is involved in protein synthesis by mTOR/S6K/S6 signaling pathway. Some S6 cDNA have been cloned in mammals in recent years but has not been identified in the goat. To facilitate such studies, we cloned the cDNA encoding Cashmere goat (Capra hircus) S6 (GenBank accession GU131122) and then detected mRNA expressi...
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