The level of virus contamination on sweetpotato germplasm resources in Ethiopia was determined. Sweetpotato (Ipomea batatus) accessions in fields at Awassa and Wondo Genet Research Stations had 85% typical virus symptoms with up to 100% incidence. The associated viruses were identified by Enzyme-linked immunosorbent assay (ELISA). Accordingly, out of 57 accessions serologically tested from Awas...

To detect five plant viruses (Beet black scorch virus, Beet necrotic yellow vein virus, Eggplant mottled dwarf virus, Pelargonium zonate spot virus, and Rice yellow mottle virus) for quarantine purposes, we designed 15 RT-PCR primer sets. Primer design was based on the nucleotide sequence of the coat protein gene, which is highly conserved within species. All but one primer set successfully amp...

2,4-Pyridinedicarboxylic acid (PDCA) is a structural analog of 2-oxoglutarate and has been shown to inhibit 2-oxoglutarate-dependent dioxygenases by competing with 2-oxoglutarate, and ethylene production in detached carnation flowers by competing with ascorbate on 1-aminocyclopropane-1-carboxylate (ACC) oxidase action. In the present study, the inhibition of ACC oxidase action by PDCA was confi...

We partially purified 1-aminocyclopropane-1-carboxylate (ACC) oxidase from senescing petals of carnation (Dianthus caryophyllus L. cv. Nora) flowers and investigated its general characteristics, and, in particular, the inhibition of its activity by ACC analogs. The enzyme had an optimum pH at 7-7.5 and required Fe2+, ascorbate and NaHCO3 for its maximal activity. The Km for ACC was calculated a...

Abstract Maize lethal necrosis (MLN) is a devastating disease of maize caused by synergistic infection with chlorotic mottle virus (MCMV) and at least one potyvirid (e.g., sugarcane mosaic virus, SCMV). MLN results in leaf necrosis, premature aging, even whole plant death can cause up to 100% losses yield. has emerged worldwide resulted serious loss production. Over the past decade, extensive r...