The possibility that structurally unrelated food additives could show either joint actions or interactions has been assessed based on their potential to share common sites and mechanisms of action or common pathways of elimination. All food additives approved in the European Union and allocated numerical acceptable daily intake values were studied, initially based on the reports by the FAO-WHO ...

A paper by Dr. G. Marsicano’s group [1] in this issue of Molecular Metabolism is clarifying and further elaborating on some of their previous results on the action of cannabinoid type 1 receptor (CB1) in neuronal mitochondria [2]. This new article was written, at least in part, in response to our finding that anti-CB1 serum used in this as well as in other studies also recognizes mitochondrial ...

BACKGROUND Accurate evaluation of immunostained histological images is required for reproducible research in many different areas and forms the basis of many clinical decisions. The quality and efficiency of histopathological evaluation is limited by the information content of a histological image, which is primarily encoded as perceivable contrast differences between objects in the image. Howe...

We describe an enzymic colourimetric method for determination of oxalate level in urine using arylamine glass-bound sorghum leaf oxalate oxidase and horseradish peroxidase. The method is based on quantification of H2O2 generated from oxidation of urinary oxalate by immobilized oxalate oxidase, by a colour reaction consisting of 4-aminophnazone, phenol and immobilized peroxidase as chromogen. Mi...

Enterobacteriaceae family comprised pathogens and commensals and has a significant impact on food safety and public health. Enterobacteriaceae is often enumerated and presumptively identified on chromogenic media, such as CHROMagar(TM) Orientation medium based on colony profile; however, classification is highly arbitrary, and some could not be differentiated due to similar chromogen production...

IN the first paper of this series an account was given of some sterol tests in which blue colours were obtained very similar in appearance to those given by arsenic trichloride or antimony trichloride with cod-liver oil, and attributed to vitamin A. On account of this close resemblance, it appeared desirable next to undertake a spectroscopic study of the "vitamin" colours with the object of dis...

Methyltestosterone (17ar-methyl-17/3-hydroxyandrost-4-ene-3one) is an androgen which is orally effective and has had wide clinical use. We have treated patients with methyltestosterone for their advanced breast cancer (1). The compound led to a substantial decrease in urinary gonad-stimulating hormone similar to that seen after the parenteral administration of testosterone propionate. However, ...

line tending to appear as a shoulder on the aspartic acid peak. Resolution could be improved, but at the expense of analysis time. To overcome these problems found with the ninhydrin method, we decided to adopt the column conditions of Callahan et al. (4), but to use a different chromogen (6, 7). The column eluate was oxidized with chloramine-T to give a pyrrole that condensed with Ehrlich’s al...

It has been suggested that achieving a chromogenic endpoint with an absorbance read at 600 nm or greater will reduce the degree of spectral interference in many colorimetric methods. We have examined a uricase/peroxidase-based system utilising a novel oxygen acceptor (azure-D2) as chromogen which produces a chromophor with an absorbance which can be measured at 600 nm (Synermed). Results (media...

Derivative spectrophotometric, colorimetric and high performance liquid chromatographic methods, for the determination of the antihistaminic cetirizine dihydrochloride in tablet form were described. Spectrophotometrically, cetirizine was determined by the measurement of its first (1D) and second (2D) derivative amplitudes at 239 (peak) and 243-233 nm (peak-to-trough), respectively. The aqueous ...