A variety of species of ruminal bacteria were screened for the ability to grow in starch-containing medium and produce amylase. Of those tested, the highest levels of amylase were produced by Streptococcus bovis JB1 and Ruminobacter amylophilus H18. Other strains that grew well on starch and produced amylase included Butyrivibrio fibrisolvens A38 and 49 and Bacteroides ruminicola 23 and B14. Va...

One of the fundamental principles of dairy nutrition is the need to formulate rations to maximize energy intake for high producing cows. We have improved our ability to predict dry matter intake (DMI) and can formulate rations for increasingly precise fractions of carbohydrate, but a major limitation to the science of carbohydrate allocation continues to be the unaccounted variability in starch...

The effect of cimetidine on normal human gastric mucus has been compared with that of carbenoxolone, a drug believed to enhance mucus production. Each drug was given for two weeks, the gastric contents aspirated over a timed period and the results assessed in unstimulated and pentagastrin stimulated secretions. The volume, dry weight and the carbohydrate contents of non-diffusable glycoconjugat...

Soil organic matter (SOM), along with the rock weathering, originating from residues of animals, plants and microorganisms involved in soil formation evolution. The stability SOM could directly produce an effect on carbon sequestration. To elucidate characteristics karst areas at molecular structure level, this study, humic acids (HA), as major proxy SOM, were extracted, purified, ultra-filtrat...

1. Mucus samples obtained from colectomy colons from 10 patients with ulcerative colitis and four patients with Crohn's disease of the colon, together with samples from 10 normal postmortem colons, have been studied. 2. Two glycoprotein fractions were isolated from mucosal scrapings by gel-permeation chromatography. The high-molecular-weight fraction consisted largely of mucus glycoproteins and...

Acid mucopolysaccharides have been extracted from whole rabbit polymorphonuclear leucocytes and from the cytoplasmic granules of these cells. The leucocyte acid mucopolysaccharides can be separated into two fractions by the solubility of their CPC complexes in solutions of differing salt concentration. One of these fractions appears to be identical with hyaluronic acid; the other appears to be ...

Lesquerella fendleri seeds contain a cross-linked carbohydrate gum with viscoelastic properties that may be used for industrial and edible applications. Laboratory studies compared the rheological behavior of gum isolates obtained from whole seed, defatted hull, and defatted meal fractions. Gum yields and rheological properties were determined for gum isolates from each of these fractions. All ...

We describe a technique for measuring carbohydrate-deficient transferrin (CDT) in serum. Serum transferrin fractions are separated by anion-exchange chromatography on microcolumns. Sialic acid-deficient transferrin fractions are collected in the eluate, and transferrin is then quantified by a rate-nephelometric technique. Imprecision (CV) was 4-5% within-run and 7-9% between runs (n = 15). Comp...