mRNA capping is a cotranscriptional event mediated by the association of capping enzyme with the phosphorylated carboxy-terminal domain (CTD) of RNA polymerase II. In the yeast Saccharomyces cerevisiae, capping enzyme is composed of two subunits, the mRNA 5'-triphosphatase (Cet1) and the mRNA guanylyltransferase (Ceg1). Here we map interactions between Ceg1, Cet1, and the CTD. Although the guan...

Fluorescent microspheres were used to measure antibody-induced capping of leukocyte membrane proteins that are immunologically related to band 3, the anion exchanger of the erythrocyte. The degree of capping was found to increase with donor age. Surface labeling and capping characteristics of cells from healthy, age-matched controls were not different from those from patients with Alzheimer's d...

I) Introduction (II) Diseases of the Pulp (III) Controversies in the Management of Exposed Pulp in the Adult Tooth NATURE OF THE CONTROVERSY POTENTIAL FACTORS OF SIGNIFICANCE TO FAILURE OF PULP CAPPING RESEARCH DIRECTIONS INDIRECT PULP CAPPING—ALTERNATIVE TO DIRECT PULP CAPPING? (IV) Controversies in Endodontic Therapy NATURE OF THE CONTROVERSY SUCCESS AND FAILURE OF ENDODONTIC THERAPY CORONAL ...

The distal ends of ciliary microtubules are attached to the membrane by microtubule-capping structures. The capping structures are located at the sites of tubulin addition and loss in vivo and may be part of the regulatory system that directs ciliary and flagellar microtubule assembly. This study describes conditions for the release and stabilization of microtubule capping structures as a first...

In lymphocytes cap formation induced by concanavalin A (con A) was found to be concentration dependent on the mitogen in the presence of colchicine, a microtubule disrupting agent. The dose-respone of cap formation under these conditions was similar to mitogen dose-response. In addition, a direct correlation was found between con A capping induced in the presence of colchicine and mitogenic res...

Direct pulp capping helps extend the life of a diseased tooth by maintaining tooth vitality. Nowadays, lasers are more frequently used during direct pulp capping in the clinic, but their use has not been previously reviewed. This review presents the basic properties of currently available lasers, scientific evidence on the effects of laser application on direct pulp capping, and future directio...

Several functional defects have been found in neutrophils from leukocyte adhesion deficiency (LAD) patients who fail to express the CD11/CD18 leukoadhesins: Mo1, LFA-1, and p150,95. To better understand the functional defects of LAD neutrophils, we have performed capping experiments. Purified normal or LAD neutrophils were labeled with fluorochrome-conjugated concanavalin A (Con A) or F(ab')2 f...