Within the large group of Estrogen Receptor alpha (ERα)-negative breast cancer patients, there is a subgroup carrying the phenotype ERα(-), PR(-), and Her2(-), named accordingly "Triple-Negative" (TN). Using cell lines derived from this TN group, we wished to establish cell clones, in which ERα is ectopically expressed, forming part of a synthetic lethality screening system. Initially, we gener...

The feeding value of extruded and nonextruded wheat and corn distillers dried grains with solubles (DDGS) for broilers was evaluated in 2 experiments. In experiment 1, male broilers (n=360) housed in battery cages were fed assay diets that included either 15 or 30% wheat or corn DDGS (extruded or not) in relation to a basal diet from d 21 to 28. Birds were killed on d 28 and ileal digesta was c...

Two lineages, epithelial, and myoepithelial cells are the main cell populations in the normal mammary gland and in breast cancer. Traditionally, cancer research has been performed using commercial cell lines, but primary cell cultures obtained from fresh breast tissue are a powerful tool to study more reliably new aspects of mammary gland biology, including normal and pathological conditions. N...

The effect of sodium and sodium salts on carcass and body organ characteristics of broilers under a four phase feeding program were investigated. A basal diet (0.08% dNa with NaCl) was formulated and one of two sources of dNa (NaHCO3 and Na2SO4) were supplemented to obtain four different percentages of dNa (0.17, 0.26, 0.35, and 0.44%) for each treatment. There was a linear decrease in dressing...

BACKGROUND PCR-based assays can improve clinical care, but they remain technically demanding and labor-intensive. We describe a new instrument, the GeneXpert, that performs automated nucleic acid isolation, reverse transcription, and fluorescence-based quantitative PCR in approximately 35 min. METHODS Yield and integrity of RNA isolated on the GeneXpert were compared with Qiagen-based extract...

The nutritive value of triticale distillers dried grains with solubles (DDGS) for broilers was investigated in 2 experiments. In experiment 1, four hundred male broilers housed in battery cages were fed diets including 15 or 30% triticale DDGS (extruded or not) or a basal diet, supplemented with or without a multi-enzyme complex from d 21 to 28. Birds were killed and ileal digesta was collected...

We investigated the effects of dietary β-1,4-mannobiose (MNB) on the weights of body, breast muscles, thighs, liver, and abdominal adipose tissue in growing broiler chicks to evaluate whether MNB can be used as a feed additive for broiler chicks. A total of 24 eight day-old male broiler chicks were allocated to two cages and fed a control diet or a 0.01% MNB-supplemented diet for 14 days. Dieta...

To identify unknown membrane proteins that could be used as targets for breast and prostate cancer immunotherapies and secreted proteins to be used as diagnostic markers, a cDNA library was generated from membrane-associated polyribosomal RNA derived from four breast cancer cell lines, one normal breast cell line, and a prostate cancer cell line. The membrane-associated polyribosomal cDNA libra...

INTRODUCTION Breast lymphoma, either as a manifestation of primary extranodal disease or as secondary involvement, is a rare malignancy, and its diagnosis, prognosis, and treatment have not been clearly defined. On the other hand, Vacuum-assisted breast biopsy (VABB) is a minimally invasive technique with ever-growing use for the diagnosis of mammographically detected, non-palpable breast lesio...