Background: The immunogenic Brucella abortus ribosomal protein L7/L12 is a promising candidate antigen for the development of subunit vaccines against brucellosis. Objective: This study was aimed to evaluate the protection of recombinant Human Serum Albumin (HAS)-L7/L12 fusion protein in Balb/c mice. Methods: The amplified L7/L12 gene was cloned in pYHSA5 vector, pYHSA5-L7/L12 construct was tra...

Bovine parvovirus has been found to infect two cell strains: buffalo lung fibroblasts and embryonic bovine tracheal cells. Infectivity titers were best determined by plaque assays. Immunofluorescence studies revealed intranuclear staining, whereas complement fixation tests confirmed bovine parvovirus antigen production. These cell strains represent economical and convenient cell types which can...

Submit Manuscript | http://medcraveonline.com Abbreviations: AIDS: Acquired Immunodeficiency Syndrome; B-cell: B lymphocyte; CTL: Cytotoxic T-lymphocyte; ELISA: Enzyme-linked Immunosorbent Assay; Env: Envelope; ER: Endoplasmic Reticulum; HIV: Human Immunodeficiency Virus; MHC: Major Histocompatibility Complex; NMR: Nuclear Magnetic Resonance; TAP: Transporter Associated with Antigen Processing;...

Submit Manuscript | http://medcraveonline.com Abbreviations: ALL: Acute Lymphoblastic Lymphoma; BLA: Biologics License Application; CAR: Chimeric Antigen Receptor; CD19: Specific Antigen; CBER: Center for Biologics Evaluation and Research; CHOP: Children’s Hospital of Philadelphia; CLL: Chronic Lymphocytic Leukemia; CRS: Cytokine Release Syndrome; CTLA: Cytotoxic T-Lymphocyte Antigen; DNA: Deox...

Lymphocyte recognition of antigen by the antigen-specific T cell receptor (TCR) and coreceptor complexes rapidly alters the cell's adhesive properties facilitating high avidity cell-ligand interactions necessary for lymphocyte development and function. Here, we report the expression of CD81 (target of antiproliferative antigen [TAPA]-1) on human thymocytes and the physical association of CD81 w...

Viral antigen prepared by heat inactivation of vaccinia virus stimulated production of interferon in association with transformation of sensitized human lymphocytes in vitro. Involvement of a macrophage-lymphocyte interaction in production of interferon stimulated by viral antigen was found in which macrophage greatly augmented the amount of interferon produced by lymphocytes. Reimmunization wi...

In this report, we describe the characterization of a novel activation antigen on porcine lymphocytes recognized by mAb 5A6/8. This antigen was detected on B and T cells 24 h after treatment with various stimuli. It was also found on alveolar macrophages, and at low levels on untreated monocytes. MAb 5A6/8 precipitated two bands of 45 and 50 kDa under non-reducing conditions, and of 22 and 28 k...

This report describes the kinetics of T-cell responses to a panel of mycobacterial antigens (PPD-M, PPD-A, ESAT-6, Ag85, 38kD, MPB64, MPB70, MPB83, hsp16.1, hsp65, and hsp70) following experimental infection of cattle with Mycobacterium bovis. Increased antigen-specific lymphocyte proliferation, gamma interferon, and interleukin-2 responses were observed in all calves following infection. Posit...

Bovine theileriosis is a tick-borne disease caused by obligate intracellular parasites related to the genus Theileria. Cellular immune responses protect cattle against pathogens through the activation of immune cells. Nowadays, live, attenuated vaccine of Theileria annulata (T. annulata) is being produced in Iran and is recommended for active cattle immunization. Detection of the immunogenic  a...