The developed RP-HPLC depends on quantitative chromatographic separation of the studied drugs on a C18 column using phosphate Buffer:acetonitrile (60:40, v/v), pH=4.6 as a mobile phase delivered at constant flow rate of 1 mL.min-1 with UV detection at 210 nm. Calibration curves for Amlodipine besylate and Perindopril Arginine were constructed over the concentration range of 1-50 μg.mL-1 for bot...

We describe a rapid, reliable "high-performance" liquid-chromatographic method of simultaneously analyzing for 4-hydroxy-3-methoxymandelic acid (I) and 4-hydroxy-3-methoxyphenylacetic acid (II) in urine. Paired-ion chromatography and amperometric detection are used in the method. A 5-mL aliquot of urine is adjusted to pH 6.1 and subjected to anion-exchange chromatography (Dowex AG 1-X4, 1.8 X 0...

migrastatin isolate contained teleocidin-related compounds which display strong inhibitory activity against tumor cell migration. In this report, we describe the effects of teleocidin-free migrastatin on tumor cell migration and on the growth of several types of tumor cells. Whenthe original migrastatin sample was subjected to HPLC (Capcell Pak C18 column, 20X250mm) developing with 70% aq aceto...

Methods: Alpha tocopherol, gamma tocopherol, and retinol are assayed using fluorescence detection. Excitation/emission wavelengths are 295 nm/330 nm and 325 nm/470 nm for the analysis of both tocopherols and retinol, respectively. Retinol acetate is employed as the internal standard. The reversed phase method incorporates gradient elution with a mobile phase consisting of methanol and acetonitr...

Methods: A simple, isocratic reverse-phase high performance liquid chromatographic (RP-HPLC) method has been developed for simultaneous estimation of methotrexate, dexamethasone and indomethacin using ibuprofen (IBP) as internal standard with photodiode array detection at 254 nm. The process was carried out on C18 column (5 μm, 250 mm X 4.6 mm) using methanol: orthophosphoric acid (1.67 % v/v) ...

Phytosterols are a group of steroids alcohols which had been regarded as a functional factor. An unknown compound in phytosterol samples and phytosterol standard samples was detected by HPLC using symmetry C18 column. The quantity of the compound was increased with the enrichment of β-sitosterol. After being collected and analyzed by GC-MS and compared with standard diagram from Wiley and Nist ...

A specific ultra-performance liquid chromatography–mass spectrometry (UPLC–MS) method was developed for the simultaneous determination of puerarin, daidzein, baicalin, wogonoside and liquiritin in rat plasma. Chromatographic separation was performed on a C18 column packed with 1.7 m particles by a linear gradient elution. The analytes and carbamazepine (internal standard, I.S.) were monitored i...

In this precise, accurate, and specific liquid-chromatographic procedure for determining progesterone in serum, the serum is diluted 10-fold with water/methanol (65/35 by vol), and the progesterone is extracted from the sample by passage through a column of graphitized carbon black (Carbopack B, Supelco). After washing the column, we elute the progesterone with chloroform/methanol (90/10 by vol...

We describe a rapid, simple HPLC method routinely used in our clinical laboratory for determining amiodarone and its metabolite desethylamiodarone. These compounds are released from serum proteins by pretreatment with an acidic solution and then extracted onto a C2 reversed-phase clean-up column. After elution from the extraction column, the compounds are separated and quantified by HPLC with a...

A reversed-phase high-performance liquid chromatographic method is described for the simultaneous determination of vitamins A, D2, D3, E and K1, retinyl acetate, retinyl palmitate, tocopherol acetate, ergosterol and 7-dehydrocholesterol in milk and butter. Narrow-bore columns are recommended because this alternative provides a good separation and efficiency, plus greater economy and sensitivity...