The importance of mesenchymal stem cells (MSC) for bone regeneration is growing. Among MSC the bone marrow-derived stem cells (BMSC) are considered the gold standard in tissue engineering and regenerative medicine; however, the adipose-derived stem cells (ASC) have very similar properties and some advantages to be considered a good alternative to BMSC. The molecular mechanisms driving adipogene...

Introduction Vascular Endothelial Growth Factor (VEGF) is involved in regulating cartilage growth and endochondral ossification during growth plate development or in cartilage degenerative processes, e.g. in osteoarthritis or following mechanical overloading. Instead, VEGF expression is suppressed during hyaline cartilage formation and maintenance. It was previously proposed that in cartilage p...

Transplantation using stem cells including bone marrow mesenchymal stem cells (BMSCs) is emerging as a potential regenerative therapy after ischemic attacks in the heart and brain. The migration capability of transplanted cells is a critical cellular function for tissue repair. Based on our recent observations that hypoxic preconditioning (HP) has multiple benefits in improving stem cell therap...

Stem cell treatment may positively influence recovery and inflammation after shock by multiple mechanisms, including the paracrine release of protective growth factors. Embryonic stem cells (ESCs) are understudied and may have greater protective power than adult bone marrow stem cells (BMSCs). We hypothesized that ESC paracrine protective mechanisms in the heart (decreased injury by enhanced gr...

The aim of the present study was to examine the effect of growth-associated protein-43 (GAP-43) on bone marrow mesenchymal stem cell (BMSC) differentiation in a rat model of traumatic optic neuropathy (TON). GAP‑43 and short hairpin (sh)RNA‑GAP‑43 were inserted into pGLV5 and pGLV3 lentiviral vectors, respectively. The stable control, GAP‑43‑overexpression and GAP‑43‑knockdown cell lines (GFP/B...

BACKGROUND Recent reports demonstrated that intravenous route, as a minimally invasive method and similar to direct injection, is suitable for bone marrow stromal cell (BMSC) transplantation. In this study, we made a comparison of intraspinal and intravenous route of BMSC administration to repair injured spinal cord tissue. METHODS Six groups of adult female rats were used in this study. Lami...

Bone is a major site of metastasis for several types of malignant tumor. Specific interactions between tumor cells and the bone microenvironment contribute to the tendency of tumors to metastasize to bone. Furthermore, Wnt5a participates in the progression of several types of malignant tumor. This study investigates the role of Wnt5a in the migration of the prostate cancer (PCa) cell line PC3 t...

MATERIAL AND METHODS Periodontal regeneration is still a challenge in terms of predictability and magnitude of effect. In this study we assess the biological effects of combining chemical root conditioning and biological mediators on three relevant cell types for periodontal regeneration. Bovine dentin slices were conditioned with 25% citric acid followed by topical application of basic fibrobl...

AIMS Randomized trial to assess change in left ventricle ejection fraction (LVEF) and myocardial perfusion in patients with acute myocardial infarction (AMI) of anterior wall treated with bone marrow stem cells (BMSCs), compared with control group-from baseline in the acute phase up to 12 months of follow-up. METHODS AND RESULTS Forty-five patients were randomized 2:1 to BMSC group (n= 31) or...

Background: Osteonecrosis of the femoral head (ONFH) is an ischemic disease characterized by impairment angiogenesis. We have previously elucidated role tsRNAs and BMSC exosomes in ONFH, but whether tsRNA-modified promote angiogenesis ONFH remains unclear. Methods: The expression angiogenesis-related tsRNA plasma from patients was examined q-PCR. function HUVECs identified CCK-8 assay. Exosomes...